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In vitro biocompatibility of solid lipid nanoparticles.
MedLine Citation:
PMID:  22750185     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This study was undertaken to address the current deficient knowledge of cellular response to solid lipid nanoparticles (SLNs) exposure. We investigated the cytotoxicity of several SLNs formulations in two fibroblast cell lineages, Vero and MDCK. Several methods were used to explore the mechanisms involved in this cytotoxic process, including cell viability assays, flow cytometry and ROS generation assessment. Among nanoparticles tested, two of them (F4 and F5) demonstrated more cytotoxic effects in both cell lineages. The cell viability assays suggested that F4 and F5 interfere in cell mitochondrial metabolism and in lysosomal activity. In addition, F5 decreased the percentage of MDCK cells in G0/G1 and G2/M phases, with a marked increase in the Sub/G1 population, suggesting DNA fragmentation. Regarding F4, although IC(50) was higher (~700μg/mL), this formulation affected mitochondrial membrane potential for Vero cells. However, the IC(50) of F5 was around 250μg/mL, suggesting the effect of SDS (sodium dodecyl sulfate) present in the formulation. In summary, the nanoparticles tested here appears to be biocompatible, with the exception of F5. Further studies are required to elucidate the in vivo effects of these nanoscale structures, in order to evaluate or predict the connotation of their increased and widespread use.
Authors:
Adny Henrique Silva; Fabíola Branco Filippin-Monteiro; Bruno Mattei; Betina G Zanetti-Ramos; Tânia Beatiz Creczynski-Pasa
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-30
Journal Detail:
Title:  The Science of the total environment     Volume:  432C     ISSN:  1879-1026     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-7-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0330500     Medline TA:  Sci Total Environ     Country:  -    
Other Details:
Languages:  ENG     Pagination:  382-388     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina P.O. Box 476, Florianópolis, SC, 88040-900, Brazil.
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