| In vitro biocompatibility of a platinum-electrode embedded photosensitive polyimide (Durimide) retinal prosthesis. | |
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MedLine Citation:
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PMID: 22775555 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Purpose: The photosensitive polyimide film, Durimide, is a common component of retinal prostheses; however, retinal cell response to Durimide has not been effectively studied This work assessed the in vitro biocompatibility of a retinal prosthesis containing platinum-electrode embedded Durimide film. Materials and methods: Biocompatibility evaluation assessed cytotoxicity, attachment, and proliferation of two cell lines: a human retinal pigmented epithelium cell line (CRL) and a rhesus monkey choroid- retinal endothelial cell line (RF/6A). Cells were cultured with the platinum-electrode embedded Durimide film, with tissue-culture treated polystyrene plates (TCPS) used as a control substrate for cell growth. The effect of a Durimide-exposed medium on cell apoptosis and life cycle was assessed using flow cytometry (FCM). Results: The indirect cytotoxicity evaluation revealed no toxic effect of the prosthesis on cells. The attachment and proliferation of CRL and RF/6A cells cultured with the Durimide prostheses showed no significant differences to the control. The FCM experiments demonstrated a liquid medium exposed to the prosthesis had no effects on apoptosis or cell life cycle in comparison with the control (p > 0.05). Conclusions: The results demonstrate that Durimide has good biocompatibility with retinal cell lines CRL and RF/6A. In conclusion, while further in vitro and in vivo studies are required to clarify long-term effects, Durimide is indicated as a promising material with suitable biocompatibility for retinal implants. |
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Authors:
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Wenjia Liu; Zhengyu Song; Yanan Mo; Weijun Wang; Xiaohong Sui; Yuanyuan Gong; Yuan Jiang; Lei Zhang; Jiannan Huang; Qing Gu; Gang Li; Chuanqing Zhou; Zhang Yu; Qiushi Ren; Xiaodong Sun; Fenghua Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-9 |
Journal Detail:
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Title: Current eye research Volume: - ISSN: 1460-2202 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Ophthalmology, Shanghai First People's Hospital Affiliated Shanghai Jiao Tong University , Shanghai , PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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