Document Detail


In vitro biocompatibility assessment of multipurpose contact lens solutions: Effects on human corneal epithelial viability and barrier function.
MedLine Citation:
PMID:  22436848     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
PURPOSE: To explore the in vitro effects of multipurpose contact lens solutions (MPSs) on corneal epithelial barrier function and viability. METHODS: Human corneal epithelial cells (HCEpiC) were exposed to 50% MPSs A-G. Viability was determined using metabolic activity, protease release and caspase assays. Barrier function was evaluated using immunostaining for the tight junction protein zonnula occludens-1 (ZO-1) and resistance measurements. RESULTS: MPS A and G did not affect HCEpiC monolayer viability after 2h, while MPSs B-F significantly decreased viability. There was a significant decrease in stratified HCEpiC viability after exposure to MPSs B-E for 2h, while there was no effect of MPS A. After exposure of HCEpiC monolayers to MPS A, F or G for 30min, ZO-1 staining appeared similar to control. HCEpiC exposed to MPSs B and C demonstrated tight junction breakdown. There was no significant change in HCEpiC monolayer resistance after exposure to MPS A or F for 2h, while MPSs B-E and G reduced resistance. After exposure to MPS A-E, stratified HCEpiC resistance was significantly decreased after 2 or 4h. The decrease in resistance was significantly less with MPS A as compared to the other MPSs. CONCLUSIONS: MPSs caused varying modifications to cell viability and barrier function in monolayer and stratified HCEpiC. MPS A did not alter monolayer HCEpiC viability or barrier function, while MPSs B-G caused significant decreases of at least one parameter. Furthermore, MPS A had significantly less effect than MPSs B-E on viability and barrier function of stratified HCEpiC.
Authors:
M E Cavet; K L Harrington; K R Vandermeid; K W Ward; J-Z Zhang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-19
Journal Detail:
Title:  Contact lens & anterior eye : the journal of the British Contact Lens Association     Volume:  -     ISSN:  1476-5411     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9712714     Medline TA:  Cont Lens Anterior Eye     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Pharmaceutical R&D, Bausch + Lomb, 1400 North Goodman Street, Rochester, NY 14609, USA.
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