Document Detail


In vitro biochemical assays to monitor rhodopsin function.
MedLine Citation:
PMID:  22688705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rhodopsin is the dim-light photoreceptor responsible for initiation of the visual transduction cascade. In the dark its activity is very low, while light activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants.
Authors:
Joshua Sammons; Alecia K Gross
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  884     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-12     Completed Date:  2012-10-19     Revised Date:  2014-05-30    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  167-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
COS Cells
Cattle
Cercopithecus aethiops
Enzyme Activation
Enzyme Assays / methods
G-Protein-Coupled Receptor Kinase 1 / metabolism
Gene Expression
In Situ Hybridization / methods
Mice
Rhodopsin / isolation & purification,  metabolism*
Transducin / isolation & purification,  metabolism
Grant Support
ID/Acronym/Agency:
T32 GM008111/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
9009-81-8/Rhodopsin; EC 2.7.11.14/G-Protein-Coupled Receptor Kinase 1; EC 3.6.1.-/Transducin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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