Document Detail

In vitro bioactivity of micro metal injection moulded stainless steel with defined surface features.
MedLine Citation:
PMID:  22562233     Owner:  NLM     Status:  In-Data-Review    
Micrometre- and nanometre-scale surface structuring with ordered topography features may dramatically enhance orthopaedic implant integration. In this study we utilised a previously optimised micron metal injection moulding (µ-MIM) process to produce medical grade stainless steel surfaces bearing micrometre scale, protruding, hemispheres of controlled dimensions and spatial distribution. Additionally, the structured surfaces were characterised by the presence of submicrometre surface roughness resulting from metal grain boundary formation. Following cytocompatibility (cytotoxicity) evaluation using 3T3 mouse fibroblast cell line, the effect on primary human cell functionality was assessed focusing on cell attachment, shape and cytoskeleton conformation. In this respect, and by day 7 in culture, significant increase in focal adhesion size was associated with the microstructured surfaces compared to the planar control. The morphological conformation of the seeded cells, as revealed by fluorescence cytoskeleton labelling, also appeared to be guided in the vertical dimension between the hemisphere bodies. Quantitative evaluation of this guidance took place using live cytoplasm fluorescence labelling and image morphometry analysis utilising both, compactness and elongation shape descriptors. Significant increase in cell compactness was associated with the hemisphere arrays indicating collective increase in focused cell attachment to the hemisphere bodies across the entire cell population. Micrometre-scale hemisphere array patterns have therefore influenced cell attachment and conformation. Such influence may potentially aid in enhancing key cellular events such as, for example, neo-osteogenesis on implanted orthopaedic surfaces.
M Bitar; V Friederici; P Imgrund; C Brose; A Bruinink
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Publication Detail:
Type:  Journal Article     Date:  2012-05-04
Journal Detail:
Title:  European cells & materials     Volume:  23     ISSN:  1473-2262     ISO Abbreviation:  Eur Cell Mater     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100973416     Medline TA:  Eur Cell Mater     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  333-47     Citation Subset:  IM    
Technical Research and Development, Novartis Pharma AG, CH-4057 Basel,
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