Document Detail

In vitro availability of calcium, iron, and zinc from first-age infant formulae and human milk.
MedLine Citation:
PMID:  11176326     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Variation in the bioavailability of calcium (Ca), iron (Fe), and zinc (Zn) occurs because of interactions of food components in the gastrointestinal microenvironment. Bioavailability is preferably determined by in vivo tests, but these are expensive, labor-intensive, time consuming, and often unethical. As an alternative, in vitro methods can be used to predict bioavailability of nutrients from foodstuffs. METHODS: A continuous-flow dialysis model with preliminary intraluminal digestive phase, adapted to the gastrointestinal conditions of infants younger than 6 months, was used. Human milk was the reference standard. Ca, Fe, and Zn content of samples and dialysates after digestion were analyzed by atomic absorption spectrometry. RESULTS: Ca availability is similar in human milk (13.1%+/-0.8%), whey (13.3%+/-1.2%), and soy-based formulae (13.0%+/-1.2%; P > 0.05), and higher in casein-predominant formula (21.2%+/-0.6%; P < 0.05). Availability of Fe is highest in human milk (8.12%+/-0.27%: P < 0.05). Fe availability in whey (1.28%+/-0.28%) and soy formulae (1.48%+/-0.28%) is similar (P > 0.05), but availability is lower in casein-predominant formula (0.48%+/-0.22%; P < 0.05). Zn availability is also highest in human milk (13.1%+/-0.7%; P < 0.05). However, Zn availability is similar in whey (6.7%+/-0.6%) and casein formulae (8.5%+/-1.6%; P > 0.05), but lower in soy formula (2.3%+/-0.4%; P < 0.05). CONCLUSIONS: Our observations are in agreement with previous data from in vivo studies in term infants. This in vitro procedure is an inexpensive, simple, rapid, and reliable method that predicts the bioavailability of Ca, Fe, and Zn in foods.
D Bosscher; M Van Caillie-Bertrand; H Robberecht; K Van Dyck; R Van Cauwenbergh; H Deelstra
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  32     ISSN:  0277-2116     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-15     Completed Date:  2001-12-04     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  54-8     Citation Subset:  IM    
Department of Pharmaceutical Sciences, University of Antwerp, Belgium.
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MeSH Terms
Biological Availability
Calcium / analysis,  pharmacokinetics*
Infant Food / analysis*
Infant Nutritional Physiological Phenomena
Infant, Newborn
Iron / analysis,  pharmacokinetics*
Micronutrients / analysis,  pharmacokinetics*
Milk Proteins / metabolism
Milk, Human / chemistry*,  metabolism
Reference Values
Reproducibility of Results
Spectrophotometry, Atomic
Zinc / analysis,  pharmacokinetics*
Reg. No./Substance:
0/Micronutrients; 0/Milk Proteins; 7439-89-6/Iron; 7440-66-6/Zinc; 7440-70-2/Calcium

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