Document Detail

In vitro augmentation of cytotoxicity by N-CWS in peritoneal polymorphonuclear leukocytes (PMNs) induced by PSK, OK-432 or N-CWS.
MedLine Citation:
PMID:  1543653     Owner:  NLM     Status:  MEDLINE    
Peritoneal polymorphonuclear leukocytes (PMNs) were collected from the peritoneal cavity of C3H/He mice 6 hrs after intraperitoneal (i.p.) injection of 2.5 mg/head of PSK, 1 KE (100 micrograms)/head of OK-432 or 200 micrograms/head of Nocardia rubra cell wall skeleton (N-CWS). Without in vitro stimulation, these PMNs did not show cytotoxicity to syngeneic MM46 mammary carcinoma cells in 51Cr release assay. Cytotoxicity of these PMNs was augmented by the addition of 25 micrograms/ml of N-CWS but not of PSK or OK-432 to cultures for the assay at the beginning of the culture. H2O2 production of PSK-induced PMNs was increased by the in vitro addition of 25 micrograms/ml of N-CWS but not of PSK. These results suggest that PSK as well as OK-432 and N-CWS can induce PMNs capable of responding further to N-CWS as the second stimulant.
H Kato; Y Yamamura; M Inoue; M Tanigawa; S Kimura; S Sugino; M Kondo
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biotherapy (Dordrecht, Netherlands)     Volume:  4     ISSN:  0921-299X     ISO Abbreviation:  Biotherapy     Publication Date:  1992  
Date Detail:
Created Date:  1992-04-13     Completed Date:  1992-04-13     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8903031     Medline TA:  Biotherapy     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-73     Citation Subset:  IM    
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
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MeSH Terms
Adjuvants, Immunologic / administration & dosage
Cell Wall / immunology
Cytotoxicity, Immunologic*
Hydrogen Peroxide / metabolism
Mice, Inbred C3H
Neutrophils / immunology*,  metabolism
Nocardia / immunology*
Picibanil / immunology
Proteoglycans / immunology
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Proteoglycans; 39325-01-4/Picibanil; 66455-27-4/krestin; 7722-84-1/Hydrogen Peroxide

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