| In vitro assessment of silver effect on porcine ovarian granulosa cells. | |
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MedLine Citation:
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PMID: 21703837 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The general objective of this in vitro study was to examine the secretory activity (insulin-like growth factor I, IGF-I) of porcine ovarian granulosa cells after Ag addition and to outline the potential intracellular mediators (cyclin B1 and caspase-3) of its effects. Ovarian granulosa cells were incubated with silver nitrate (AgNO(3)) at the doses 0.09, 0.17, 0.33, 0.5 and 1.0mg/mL for 18h and compared to the control group without metal addition. The release of IGF-I by granulosa cells was assessed by RIA and expression of cyclin B1 and caspase-3 immunocytochemistry. Our observations show that IGF-I release by granulosa cells was significantly (P<0.05) stimulated by AgNO(3) addition at the doses (0.09-1.0mg/mL). Similarly to IGF-I the cyclin B1 and caspase-3 expression in ovarian granulosa cells was stimulated by Ag addition (0.09-1.0mg/mL). In conclusion, the present results indicate, a direct effect of Ag on (1) secretion of growth factor IGF-I, (2) expression of markers of proliferation (cyclin B1) and apoptosis (caspase-3) of porcine ovarian granulosa cells and (3) that the effect of Ag on ovarian cell proliferation could be mediated by IGF-I and cyclin B1. Obtained data indicate the interference of Ag in the pathways of proliferation and apoptosis of porcine ovarian granulosa cells through hormonal and intracellular peptides such as are cyclin B1 and caspase-3. |
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Authors:
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Adriana Kolesarova; Marcela Capcarova; Alexander V Sirotkin; Marina Medvedova; Jaroslav Kovacik |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-22 |
Journal Detail:
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Title: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) Volume: - ISSN: 1878-3252 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-6-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9508274 Medline TA: J Trace Elem Med Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier GmbH. All rights reserved. |
Affiliation:
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Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovak Republic. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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