| In vitro assessment of iron on porcine ovarian granulosa cells: secretory activity, markers of proliferation and apoptosis. | |
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MedLine Citation:
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PMID: 21401293 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The obtained data could expand the existing general knowledge concerning direct action of metals on the ovary. Nevertheless, the results of testing of iron compound on porcine ovarian cells should be interpreted carefully because iron is an essential element but on the other hand it could induce changes in cellular processes. The general objective of this in vitro study was to first examine dose-dependent effects of iron on the secretory activity of porcine ovarian granulosa cells, secondly to outline the potential intracellular mediators that mediate these effects. Specifically, we evaluated the effect of FeSO(4).7H(2)O on the release of insulin-like growth factor I (IGF-I) and progesterone, as well as the expression of markers of proliferation (cyclin B1) and apoptosis (caspase-3) in porcine ovarian granulosa cells. Concentrations of IGF-I and progesterone were determined by RIA, cyclin B1 and caspase-3 expression by immunocytochemistry (ICC). Our results show a significantly decreased IGF-I secretion by ovarian granulosa cells after Fe addition at the doses 0.5 and 1.0 mg/ml. The iron additions at doses 0.17 and 1.0 mg/ml had no effect on progesterone secretion. In contrast, iron addition at doses 0.17 and 1.0 mg/ml, resulted in stimulation of cyclin B1 and caspase -3 expression. In conclusion, the present results indicate, (1) a direct effect of Fe on secretion of growth factor IGF-I but not steroid hormone progesterone, (2) expression of markers of proliferation (cyclin B1) and (3) apoptosis (caspase-3) of porcine ovarian granulosa cells. These results support an idea that iron could play a regulatory role in porcine ovarian function: hormone release, proliferation and apoptosis. |
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Authors:
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A Kolesarova; M Capcarova; M Medvedova; A Sirotkin; J Kovacik |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-14 |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: - ISSN: 1802-9973 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic. adrianakolesarova@yahoo.com, Adriana.Kolesarova@uniag.sk. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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