Document Detail

In vitro assays for detection of diphtheria toxin.
MedLine Citation:
PMID:  20692989     Owner:  NLM     Status:  In-Data-Review    
Subunit-specific antipeptide antibodies have been used to develop a dot blot immunoassay for detection of diphtheria toxin (DT) produced by toxigenic strains of Corynebacterium diphtheriae. A detection limit of 0.1-1.0 ng DT/ml was obtained. DT is cytotoxic to cultured Vero cells and cell viability if conveniently assessed using a tetrazolium dye, MTT. This has been used as the basis of a cytotoxicity assay for DT, which has a detection limit of 20 pg/ml of biologically active toxin. Sixty isolates of C. diphtheriae were assayed for toxigenicity. There was complete concordance between the Vero cell cytotoxicity assay and guinea pig intradermal assay in vivo, demonstrating the utility of the cytotoxicity assay.
C S Hoy; D Sesardic
Related Documents :
12353869 - Microcystins in natural blooms and laboratory cultured microcystis aeruginosa from lagu...
24263909 - The freudian superego.
1925309 - Production of a cytotoxin by helicobacter pylori.
4866099 - Intracellular synthesis of clostridium botulinum type b toxin. i. demonstration of toxi...
3660579 - Purification of a heat labile dermonecrotic toxin from culture fluid of pasteurella mul...
21154679 - Influence of pore size on the redifferentiation potential of human articular chondrocyt...
7968379 - 1,25-dihydroxyvitamin d3 regulates the synthesis of nerve growth factor in primary cult...
23604699 - Behaviour of co-cultured human osteoclastic and osteoblastic cells exposed to endodonti...
21228359 - In situ hybridization for specific fungal organisms in acute invasive fungal rhinosinus...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  8     ISSN:  0887-2333     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  1994 Aug 
Date Detail:
Created Date:  2010-08-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  693-5     Citation Subset:  -    
Division of Bacteriology, National Institute for Biological Standards and Control, South Mimms, Hertfordshire EN6 3QG, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Use of in vitro methodology to predict the irritancy potential of surfactants.
Next Document:  The stem-cell test-A novel in vitro assay for teratogenic potential.