Document Detail

In vitro assays for botulinum toxin and antitoxins.
MedLine Citation:
PMID:  3792644     Owner:  NLM     Status:  MEDLINE    
Clostridium botulinum produces several powerful neuroparalytic toxins which, although rare in food-poisoning instances, are generally fatal. A considerable amount of effort has therefore been made by the food industry to ensure that food treatment processes adequate to prevent growth and toxin production by Cl. botulinum. Laboratory mice and guinea-pigs are presently used extensively both for the assay of botulinum toxins and for the development and assessment of vaccines used to protect laboratory workers. An amplified ELISA, using a monoclonal antibody, has been developed for botulinum type A toxin with a sensitivity similar to that of the mouse acute toxicity test. The immunoassay has been found to be applicable to the detection of toxin in foodstuffs and could replace the currently used mouse bioassay in many laboratories. Immunoassays have also been developed for the detection of antibodies to botulinum toxins. A preliminary study has shown that antibody titres to botulinum types A and B toxins in sera taken from immunised personnel, as measured by ELISA, showed limited correlation with those measured by the toxin neutralisation test in mice. A more extensive study should determine whether the latter test can be replaced by the ELISA.
C Shone; N Appleton; P Wilton-Smith; P Hambleton; N Modi; S Gatley; J Melling
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Developments in biological standardization     Volume:  64     ISSN:  0301-5149     ISO Abbreviation:  Dev. Biol. Stand.     Publication Date:  1986  
Date Detail:
Created Date:  1987-02-02     Completed Date:  1987-02-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0427140     Medline TA:  Dev Biol Stand     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  141-5     Citation Subset:  IM    
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MeSH Terms
Animal Testing Alternatives
Antibodies, Monoclonal / diagnostic use
Botulinum Antitoxin / analysis*
Botulinum Toxins / analysis*
Enzyme-Linked Immunosorbent Assay / methods
Food Microbiology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Botulinum Antitoxin; 0/Botulinum Toxins

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