| In vitro assay for protein digestibility: interlaboratory study. | |
| | |
MedLine Citation:
|
PMID: 2211487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
True protein digestibilities of 17 protein sources were estimated by 6 laboratories using an in vitro, 3-enzyme digestion system in a pH stat. Samples from animal, vegetable, and mixed food sources were freeze-dried (if not already dried), ground, mixed, and shipped to each collaborator along with a sodium caseinate standard and trypsin, chymotrypsin, and peptidase. The uptake of titrant during enzymatic digestion was used to calculate estimates of digestibility. Digestibilities ranged from 100% for casein to 89.9% for whole wheat cereal. Mean relative standard deviations for repeatability were 1.4% for rolled oats and less than 1% for the remaining 16 samples. Mean relative standard deviations for reproducibility ranged from 5.0 to 0.8%; values were less than 2.5% for 13 of the 17 samples. |
| | |
Authors:
|
F E McDonough; G Sarwar; F H Steinke; P Slump; S Garcia; S Boisen |
Related Documents
:
|
2130147 - Effect of dietary proteins and/or their digestive products on intestinal taurocholate a... 15261027 - A dynamic wicking technique for determining the effective pore radius of pregelatinized... 8146107 - Resistant starch content of indian foods. 15054247 - Combined steam pretreatment and enzymatic hydrolysis of starch-free wheat fibers. 15298187 - Physical and kinetic speciation of copper and zinc in three geochemically contrasting m... 2641487 - Detection of staphylococcal enterotoxins in dairy products by the reversed passive late... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal - Association of Official Analytical Chemists Volume: 73 ISSN: 0004-5756 ISO Abbreviation: J Assoc Off Anal Chem Publication Date: 1990 Jul-Aug |
Date Detail:
|
Created Date: 1990-11-02 Completed Date: 1990-11-02 Revised Date: 2004-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 7505559 Medline TA: J Assoc Off Anal Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 622-5 Citation Subset: IM |
Affiliation:
|
U.S. Department of Agriculture, Beltsville Human Nutrition Research Center, Beltsville, MD 20705. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Caseins / analysis Chymotrypsin / diagnostic use Dietary Proteins / analysis* Egg Proteins / analysis Hydrogen-Ion Concentration Meat / analysis Nitrogen / analysis Peptide Hydrolases / diagnostic use Trypsin / diagnostic use Vegetables / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Caseins; 0/Dietary Proteins; 0/Egg Proteins; 7727-37-9/Nitrogen; EC 3.4.-/Peptide Hydrolases; EC 3.4.21.1/Chymotrypsin; EC 3.4.21.4/Trypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Flame atomic absorption spectrometric determination of serum zinc: collaborative study.
Next Document: Determination of taurine in infant formulas using ultrafiltration and cation-exchange chromatography...