Document Detail

In vitro approach to 'uremic cardiomyopathy'.
MedLine Citation:
PMID:  8289989     Owner:  NLM     Status:  MEDLINE    
Cardiovascular complications determine the prognosis of patients with chronic renal failure. The contribution of compounds retained during uremia to specific myocardial lesions is controversial. We investigated the contractility of spontaneously beating mouse cardiac myocytes in culture under perfusion with sera derived from patients on maintenance hemodialysis and test solutions containing possible toxins. Cellular contractility under defined environmental conditions is determined by a computer-assisted digital image analysis. 'Uremic sera', creatinine, urea, and combinations of these compounds reduce inotropy of the cultured heart cells, induce arrhythmias or asynchronies in a concentration-dependent manner. We propose the myocyte perfusion technique as an in vitro approach to identify cardiotoxins in the body fluids of chronically uremic patients.
D Weisensee; I Löw-Friedrich; M Riehle; J Bereiter-Hahn; W Schoeppe
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nephron     Volume:  65     ISSN:  0028-2766     ISO Abbreviation:  Nephron     Publication Date:  1993  
Date Detail:
Created Date:  1994-02-22     Completed Date:  1994-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0331777     Medline TA:  Nephron     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  392-400     Citation Subset:  IM    
Cinematic Cell Research Group, Hospital of the Johann Wolfgang Goethe-University, Frankfurt am Main, FRG.
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MeSH Terms
Cardiomyopathies / etiology*
Cells, Cultured
Creatinine / pharmacology
Models, Cardiovascular
Myocardial Contraction / drug effects
Myocardium / cytology
Toxins, Biological / metabolism,  toxicity
Urea / pharmacology
Uremia / complications*,  metabolism
Reg. No./Substance:
0/Toxins, Biological; 57-13-6/Urea; 60-27-5/Creatinine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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