Document Detail

In vitro antioxidant synergism and antagonism between food extracts can lead to similar activities in H(2) O(2) -induced cell death, caspase-3 and MMP-2 activities in H9c2 cells.
MedLine Citation:
PMID:  22538730     Owner:  NLM     Status:  Publisher    
BACKGROUND: The cardio-health-promoting activity of some foods may be due to their specific antioxidant content. The antioxidant activity of a mixture of plant extracts has been shown to differ from the activity of the individual extracts. As a result, the activity of the mixture can be described as synergistic, antagonistic or additive. This in vitro study evaluated the relationship between the in vitro antioxidant capacity of mixtures and their bioactivity when cardiomyocytes (H9c2) were challenged with H(2) O(2) . RESULTS: A mixture of raspberry and adzuki bean extracts produced a synergistic response and a mixture of broccoli and soybean extracts produced an antagonistic response in chemical-based antioxidant assays. When these extracts were tested in cell cultures, individually and in mixtures, the mixture of raspberry and adzuki bean protected the cardiomyocytes from H(2) O(2) -induced cell damage significantly better than the individual extracts. Conversely, the mixture of broccoli and soybean extracts was less effective in protecting H9c2 cells. The synergistic and antagonistic effects of the mixtures in protecting cell damage were brought about by enhanced or reduced ability in attenuating caspase-3 and matrix metalloproteinase-2 activities elevated by H(2) O(2) . CONCLUSION: Food mixtures with synergistic antioxidant activity and protective property against reactive oxygen species-induced cell death can potentially be incorporated into novel functional foods or beverages with optimum health benefit. The antagonistic effect of food mixtures can be a health concern and thus should be avoided. Copyright © 2012 Society of Chemical Industry.
Sunan Wang; Kelly A Meckling; Massimo F Marcone; Yukio Kakuda; Amy Proulx; Rong Tsao
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-26
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  -     ISSN:  1097-0010     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Society of Chemical Industry.
Guelph Food Research Center, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, N1G 5C9, Canada; Department of Food Science, University of Guelph, Guelph, Ontario, N1G 2 W1, Canada.
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