| In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena. | |
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MedLine Citation:
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PMID: 8423249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Calprotectin is a complex of two anionic proteins found in abundance in the cytosol of neutrophils, certain macrophages, and oral epithelial keratinocytes. Bacteria of the genus Capnocytophaga are pathogens of periodontal origin which can cause systemic infection in neutropenic subjects. Recently, it has been observed that Capnocytophaga may be internalized by neutrophils within the cytosol rather than within a membrane-delimited phagosome. The purpose of this study was to test the in vitro antibacterial effect of the cytosolic complex, calprotectin, against Capnocytophaga sputigena. Calprotectin was purified from the cytosol of human neutrophils by gel filtration and anion exchange FPLC, and it exerted potent in vitro antimicrobial effects against C. sputigena. Net bacteriostatic activity was exerted up to 18 h, after which bactericidal effects were observed. Both net bacteriostatic and bactericidal activity occurred at concentrations above 20 micrograms/mL and exhibited identical dose-response characteristics. Particle counts increased in the presence of calprotectin, despite net bacteriostasis as assessed by changes in colony-forming units (CFU). Dose-response characteristics and direct particle counts suggested that net bacteriostatic effects were the result of balanced cell division and death, rather than suspension of cell division. We conclude that calprotectin can be a significant contributor to host defense against infection by Capnocytophaga. |
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Authors:
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K T Miyasaki; A L Bodeau; A R Murthy; R I Lehrer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of dental research Volume: 72 ISSN: 0022-0345 ISO Abbreviation: J. Dent. Res. Publication Date: 1993 Feb |
Date Detail:
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Created Date: 1993-02-24 Completed Date: 1993-02-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 517-23 Citation Subset: D; IM |
Affiliation:
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Section of Oral Biology, UCLA School of Dentistry 90024-1668. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Anti-Bacterial Agents / pharmacology* Capnocytophaga / drug effects* Cell Adhesion Molecules, Neuronal / chemistry, isolation & purification, pharmacology* Colony Count, Microbial Cytosol / chemistry Electrophoresis, Polyacrylamide Gel Immunoblotting Leukocyte L1 Antigen Complex Microbial Sensitivity Tests Molecular Sequence Data S100 Proteins / physiology |
| Grant Support | |
ID/Acronym/Agency:
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AI 25693/AI/NIAID NIH HHS; DE 08161/DE/NIDCR NIH HHS; DE00282/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Cell Adhesion Molecules, Neuronal; 0/Leukocyte L1 Antigen Complex; 0/S100 Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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