| In vitro anti-fibrotic activities of herbal compounds and herbs. | |
| | |
MedLine Citation:
|
PMID: 19474275 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: We recently developed high-throughput assays of inflammation-independent anti-fibrotic activities based on TGF-beta1-induced total collagen accumulation and nodule formation in normal rat kidney fibroblasts. METHODS: These assays were applied to examine the anti-fibrotic activities of 21 compounds isolated from plants used in Chinese medicine and methanol extracts of 12 Chinese herbs. Lactate dehydrogenase release assay and cell detachment index were used to monitor cytotoxicity. Changes in fibrogenic molecular markers were observed by reverse transcriptase quantitative polymerase chain reaction and high-content imaging analysis of immunofluorescence. RESULTS: Three flavonoids (quercetin, baicalein and baicalin) and two non-flavonoids (salvianolic acid B and emodin) demonstrated anti-fibrotic activities in both total collagen accumulation and nodule formation assays. The remaining 16 compounds had little anti-fibrotic effect or were cytotoxic. The anti-fibrotic compounds suppressed collagen I expression at both mRNA and protein levels and also variably suppressed alpha-smooth muscle actin expression and bromodeoxyuridine incorporation. Methanol extracts of Scutellaria baicalensis Georgi, Salvia miltiorrhiza Bunge and Rheum palmatum L., which are rich sources of baicalein, baicalin, salvianolic acid B and emodin, respectively, also showed in vitro anti-fibrotic activities. CONCLUSIONS: Five herbal compounds and three herbal extracts have in vitro anti-fibrotic activities. These data warrant further studies on these anti-fibrotic entities and suggest it a promising strategy to discover new anti-fibrotic drugs by screening more plant materials. |
| | |
Authors:
|
Qin Hu; Mazhar Noor; Yuen Fei Wong; Peter J Hylands; Monique S J Simmonds; Qing Xu; Dan Jiang; Bruce M Hendry; Qihe Xu |
Related Documents
:
|
18690825 - Clinical status of anti-cancer agents derived from marine sources. 18165185 - Synthesis and biological evaluation of thieno[2,3-d]pyrimidine derivatives for anti-inf... 10437135 - Relationship between structure and anti-oxidation of tocopherol with molecular orbit th... 16707845 - Inhibitory action of eugenol compounds on the production of nitric oxide in raw264.7 ma... 985795 - Effect of long-chain acyl-coa on the omega-oxidation of monocarboxylic acid. 20183315 - A novel norditerpenoid alkaloid from aconitum macrorhynchum. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-27 |
Journal Detail:
|
Title: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 24 ISSN: 1460-2385 ISO Abbreviation: Nephrol. Dial. Transplant. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-22 Completed Date: 2009-12-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8706402 Medline TA: Nephrol Dial Transplant Country: England |
Other Details:
|
Languages: eng Pagination: 3033-41 Citation Subset: IM |
Affiliation:
|
Department of Renal Medicine, King's College London, London, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cells, Cultured Fibrosis / prevention & control* Plant Preparations / therapeutic use* Rats |
| Chemical | |
Reg. No./Substance:
|
0/Plant Preparations |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The predictive performance of plasma neutrophil gelatinase-associated lipocalin (NGAL) increases wit...
Next Document: Congenital disorders of glycosylation: a rare cause of nephrotic syndrome.