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In vitro angiogenesis on the human amniotic membrane: requirement for basic fibroblast growth factor-induced proteinases.
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MedLine Citation:
PMID:  2465298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of basic fibroblast growth factor-(bFGF) induced proteinases in basement membrane (BM) invasion by bovine capillary endothelial (BCE) cells was studied using a quantitative in vitro assay previously described (Mignatti et al., 1986). 125I-iododeoxyuridine-labeled BCE cells were grown for 72 h on the human amnion BM, and cell invasion was determined by measuring the radioactivity associated with the tissue after removal of the noninvasive cell layer. BCE cells were noninvasive under normal conditions. Addition of human bFGF to either the BM or to the stromal aspect of the amnion induced BCE cell invasion with a dose-dependent response. This effect was maximal in the presence of 70 ng/ml bFGF, and was inhibited by anti-FGF antibody. Transforming growth factor beta, as well as plasmin inhibitors and anti-tissue type plasminogen activator antibody inhibited BCE cell invasion. The tissue inhibitor of metalloproteinases, 1-10 phenanthroline, anti-type IV and anti-interstitial collagenase antibodies had the same effect. On the contrary, anti-stromelysin antibody and Eglin, an inhibitor of elastase, were ineffective. The results obtained show that both the plasminogen activator-plasmin system and specific collagenases are involved in the invasive process occurring during angiogenesis.
Authors:
P Mignatti; R Tsuboi; E Robbins; D B Rifkin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  108     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1989-03-28     Completed Date:  1989-03-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  671-82     Citation Subset:  IM    
Affiliation:
Department of Cell Biology, New York University School of Medicine, New York 10016.
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MeSH Terms
Descriptor/Qualifier:
Amnion / blood supply*,  cytology
Animals
Basement Membrane / blood supply,  cytology
Cattle
Cell Adhesion
Endopeptidases / metabolism*
Endothelium, Vascular / cytology*
Fibrinolysin / antagonists & inhibitors
Fibroblast Growth Factors / pharmacology*
Humans
Idoxuridine / metabolism
Metalloendopeptidases / antagonists & inhibitors
Neovascularization, Pathologic
Protease Inhibitors / pharmacology
Transforming Growth Factors / pharmacology
Chemical
Reg. No./Substance:
0/Protease Inhibitors; 54-42-2/Idoxuridine; 62031-54-3/Fibroblast Growth Factors; 76057-06-2/Transforming Growth Factors; EC 3.4.-/Endopeptidases; EC 3.4.21.7/Fibrinolysin; EC 3.4.24.-/Metalloendopeptidases
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 2 Year: 1989
Volume: 108 Issue: 2
First Page: 671 Last Page: 682
ID: 2115414
Publisher Id: 89139584
PubMed Id: 2465298

In vitro angiogenesis on the human amniotic membrane: requirement for basic fibroblast growth factor-induced proteinases


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