| In vitro analysis of radiation-induced dermal wounds. | |
| | |
MedLine Citation:
|
PMID: 20493356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To investigate the pathophysiology of radiation-induced wounds of the head and neck at a molecular level. STUDY DESIGN: Basic science, prospective study. SETTING: The study was conducted at the Department of Otolaryngology-Head and Neck Surgery, Ruprecht Karls-University Heidelberg, Faculty of Medicine Mannheim, Mannheim, Germany. SUBJECTS AND METHODS: Keratinocytes from chronic nonhealing ulcers in irradiated areas as well as from healthy skin areas in the same patients (n = 3) were harvested during surgical procedures and isolated in cell culture. First, a proliferation assay was performed. Gene expression was analyzed by microarray, protein expression by immunohistochemistry. RESULTS: Keratinocytes from radiogenic wounds showed a shift from the high molecular keratins 1 and 10 to the low molecular keratins 5 and 14 compared to normal control skin. Keratinocytes from nonhealing wounds showed a decreased expression of transforming growth factor alpha and beta 1, fibroblast growth factor 1 and 2, keratinocyte growth factor, vascular endothelial growth factor, and hepatocyte growth factor. The matrix metalloproteinases 2, 12, and 13 showed increased expression in irradiated keratinocytes and fibroblasts. CONCLUSION: Our data showed a change of keratinocytes to a less differentiated state due to radiation. Additionally, it seems that radiation-induced dermal injuries often fail to heal because of decreased proliferation, impaired angiogenesis, and persistently high concentrations of matrix metalloproteinases. |
| | |
Authors:
|
Ulrich Reinhart Goessler; Peter Bugert; Stefan Kassner; Jens Stern-Straeter; Gregor Bran; Haneen Sadick; Karl H?rmann; Frank Riedel |
Related Documents
:
|
10729786 - Identification of epidermal growth factor mrna-expressing cells in the mouse anterior p... 16274956 - Variations in the mrna expression of inflammatory mediators, markers of differentiation... 7909356 - Oct-6: a regulator of keratinocyte gene expression in stratified squamous epithelia. 15086536 - The raig family member, gprc5d, is associated with hard-keratinized structures. 9988706 - Multiple functional sp1 domains in the minimal promoter region of the neuronal nicotini... 20664976 - Anril is implicated in the regulation of nucleus and potential transcriptional target o... |
Publication Detail:
|
Type: Journal Article Date: 2010-04-24 |
Journal Detail:
|
Title: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery Volume: 142 ISSN: 1097-6817 ISO Abbreviation: Otolaryngol Head Neck Surg Publication Date: 2010 Jun |
Date Detail:
|
Created Date: 2010-05-24 Completed Date: 2010-06-10 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8508176 Medline TA: Otolaryngol Head Neck Surg Country: United States |
Other Details:
|
Languages: eng Pagination: 845-50 Citation Subset: IM |
Copyright Information:
|
Copyright 2010 American Academy of Otolaryngology-Head and Neck Surgery Foundation. Published by Mosby, Inc. All rights reserved. |
Affiliation:
|
Department of Otolaryngology-Head and Neck Surgery, Ruprecht Karls-University Heidelberg, Faculty of Medicine Mannheim, Mannheim, Germany. ugoessler@web.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carcinoma, Squamous Cell
/
radiotherapy,
surgery Cell Differentiation / radiation effects Cumulus Cells Cytokines / physiology Head and Neck Neoplasms / radiotherapy, surgery Humans Immunohistochemistry Keratinocytes / physiology*, radiation effects* Matrix Metalloproteinases / analysis Oligonucleotide Array Sequence Analysis Prospective Studies Radiation Injuries / physiopathology* Skin / radiation effects Soft Tissue Injuries / physiopathology Ulcer / physiopathology* Wound Healing / physiology* |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; EC 3.4.24.-/Matrix Metalloproteinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Time-resolved laser-induced fluorescence spectroscopy as a diagnostic instrument in head and neck ca...
Next Document: Role of thyroidectomy in advanced laryngeal and pharyngolaryngeal carcinoma.