Document Detail


In vitro analysis of radiation-induced dermal wounds.
MedLine Citation:
PMID:  20493356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the pathophysiology of radiation-induced wounds of the head and neck at a molecular level. STUDY DESIGN: Basic science, prospective study. SETTING: The study was conducted at the Department of Otolaryngology-Head and Neck Surgery, Ruprecht Karls-University Heidelberg, Faculty of Medicine Mannheim, Mannheim, Germany. SUBJECTS AND METHODS: Keratinocytes from chronic nonhealing ulcers in irradiated areas as well as from healthy skin areas in the same patients (n = 3) were harvested during surgical procedures and isolated in cell culture. First, a proliferation assay was performed. Gene expression was analyzed by microarray, protein expression by immunohistochemistry. RESULTS: Keratinocytes from radiogenic wounds showed a shift from the high molecular keratins 1 and 10 to the low molecular keratins 5 and 14 compared to normal control skin. Keratinocytes from nonhealing wounds showed a decreased expression of transforming growth factor alpha and beta 1, fibroblast growth factor 1 and 2, keratinocyte growth factor, vascular endothelial growth factor, and hepatocyte growth factor. The matrix metalloproteinases 2, 12, and 13 showed increased expression in irradiated keratinocytes and fibroblasts. CONCLUSION: Our data showed a change of keratinocytes to a less differentiated state due to radiation. Additionally, it seems that radiation-induced dermal injuries often fail to heal because of decreased proliferation, impaired angiogenesis, and persistently high concentrations of matrix metalloproteinases.
Authors:
Ulrich Reinhart Goessler; Peter Bugert; Stefan Kassner; Jens Stern-Straeter; Gregor Bran; Haneen Sadick; Karl H?rmann; Frank Riedel
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Publication Detail:
Type:  Journal Article     Date:  2010-04-24
Journal Detail:
Title:  Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery     Volume:  142     ISSN:  1097-6817     ISO Abbreviation:  Otolaryngol Head Neck Surg     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-06-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8508176     Medline TA:  Otolaryngol Head Neck Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  845-50     Citation Subset:  IM    
Copyright Information:
Copyright 2010 American Academy of Otolaryngology-Head and Neck Surgery Foundation. Published by Mosby, Inc. All rights reserved.
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Ruprecht Karls-University Heidelberg, Faculty of Medicine Mannheim, Mannheim, Germany. ugoessler@web.de
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / radiotherapy,  surgery
Cell Differentiation / radiation effects
Cumulus Cells
Cytokines / physiology
Head and Neck Neoplasms / radiotherapy,  surgery
Humans
Immunohistochemistry
Keratinocytes / physiology*,  radiation effects*
Matrix Metalloproteinases / analysis
Oligonucleotide Array Sequence Analysis
Prospective Studies
Radiation Injuries / physiopathology*
Skin / radiation effects
Soft Tissue Injuries / physiopathology
Ulcer / physiopathology*
Wound Healing / physiology*
Chemical
Reg. No./Substance:
0/Cytokines; EC 3.4.24.-/Matrix Metalloproteinases

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