| In vitro amplification techniques for the detection of nucleic acids: new tools for the diagnostic laboratory. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 2672619 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The acceptance of nucleic acid probes as diagnostic tools for the clinical laboratory has been hampered by a number of factors, including laborious techniques and limited sensitivity. The focus of this review is on the recent development of amplification techniques to enhance the signal generated by nucleic acid-based detection systems. Three general areas are discussed: (1) amplification of target sequences using the polymerase chain reaction or the transcript amplification system, (2) amplification of the probe sequences using Q beta replicase, and (3) amplification of probe-generated signals with compound or "Christmas tree" probes. The hope of these new technologies is to simplify yet improve on the sensitivity of nucleic acid-based tests to enable them to attain a more prominent place in the diagnostic repertoire of the clinical laboratory. |
| | |
Authors:
|
D H Persing; M L Landry |
Related Documents
:
|
6195809 - Nucleic acid spectrophotometry on paper disks. 2425659 - Nucleic acid quantitation by continuous flow fluorometry. 11754339 - Development and current status of the charmm force field for nucleic acids. 11688739 - Topography, structure and function of the patella and the patelloid in marsupials. 9806459 - Simple and broadly applicable sample preparation by use of magnetic glass particles. 8013949 - Nucleic acid amplification and infectious disease. 6870859 - Metabolic fate of non-esterified fatty acids in isolated hepatocytes from newborn and y... 8067689 - Dietary lipids from marine unicellular algae enhance the amount of liver and blood omeg... 7106189 - Possible roles of central cholinergic nicotinic mechanisms in regulation of gastric fun... |
Publication Detail:
|
Type: Journal Article; Review |
Journal Detail:
|
Title: The Yale journal of biology and medicine Volume: 62 ISSN: 0044-0086 ISO Abbreviation: Yale J Biol Med Publication Date: 1989 Mar-Apr |
Date Detail:
|
Created Date: 1989-09-26 Completed Date: 1989-09-26 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0417414 Medline TA: Yale J Biol Med Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 159-71 Citation Subset: IM |
Affiliation:
|
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals DNA, Viral / analysis* Humans Molecular Biology / methods Nucleic Acid Amplification Techniques* Q beta Replicase / metabolism RNA, Viral / analysis* Virus Diseases / diagnosis* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Viral; 0/RNA, Viral; EC 2.7.7.-/Q beta Replicase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): Yale J Biol Med Journal ID (pmc): yjbm ISSN: 0044-0086 ISSN: 1551-4056 Publisher: Yale Journal of Biology and Medicine |
Article Information Download PDF  Print publication date: Season: Mar?Apr Year: 1989 Volume: 62 Issue: 2 First Page: 159 Last Page: 171 ID: 2589213 PubMed Id: 2672619 |
| In vitro amplification techniques for the detection of nucleic acids: new tools for the diagnostic laboratory. | |
| D. H. Persing | |
| M. L. Landry | |
| Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510. |
|
Article Categories:
|
|
Previous Document: New prospects for the diagnosis of viral infections.
Next Document: The impact of cell culture sensitivity on rapid viral diagnosis: a historical perspective.