Document Detail


In vitro adult rat adipose tissue-derived stromal cell isolation and differentiation.
MedLine Citation:
PMID:  21082393     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Mesenchymal stromal cell research has advanced significantly over the last decade, especially in the area of adult adipose-derived stem cells (ASCs). There are significant species differences in ASC harvest, as well as in vitro doubling time and differentiation. Selection of appropriate animal models for preclinical evaluations is critical for optimization and validation of ASC therapeutic effects. As such, in vitro studies are necessary precursors to in vivo ASC applications. Additionally, to elucidate environmental, disease, or trauma effects on native stromal cell populations, characterization of cells from unexposed individuals is necessary. Rats are an established immunocompetent small animal model for a large number of human disorders and toxin exposures in the majority of organ systems. Isolation and expansion techniques for rat ASCs continue to change with advances in technology and ASC biology. The information presented in this chapter is a summary of rat ASC harvest, isolation, quantification, and in vitro expansion.
Authors:
Mandi J Lopez; Nakia D Spencer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  702     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2011  
Date Detail:
Created Date:  2010-11-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-46     Citation Subset:  IM    
Affiliation:
Laboratory for Equine and Comparative Orthopedic Research, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
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Descriptor/Qualifier:
Grant Support
ID/Acronym/Agency:
R15 AA017546/AA/NIAAA NIH HHS

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