Document Detail

In vitro adipocytic conversion in Meckel's chondrocytes in response to a fatty acid-containing medium.
MedLine Citation:
PMID:  17031022     Owner:  NLM     Status:  MEDLINE    
Chick serum (CKS) contains factors that stimulate adipocytes in Meckel's chondrocytes in vitro. In the present study, we analyzed levels of fatty acids in CKS, and further examined whether these had the potential to convert chondrocytes to adipocytes. Phenotypic changes were evaluated by light and electron microscopies, bromodeoxyuridine (BrdU) incorporation, triglyceride assays, and immunocytochemistry. We showed that CKS contained high levels of fatty acids, and a mixed medium containing 5 particular fatty acids inhibited DNA synthesis and the proliferation of chondrocytes as it facilitated their differentiation into adipocytes. The adipocytes produced were sudan-positive multilocular cells that morphologically and histochemically resembled adipocytes induced by the CKS-containing medium. Almost all lipid droplet-containing cells were positive for leptin and alpha-glycerophosphate dehydrogenase (GPDH), as evaluated by immunoperoxidase staining, and their triglyceride concentrations markedly increased during 4 to 6 days of culture. These results suggested that specific fatty acids in CKS are involved in the adipocytic conversion of Meckel's chondrocytes.
Kiyoto Ishizeki; Tadayoshi Kagiya; Naoki Fujiwara; Hidemitsu Harada
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of histology and cytology     Volume:  69     ISSN:  0914-9465     ISO Abbreviation:  Arch. Histol. Cytol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-10-10     Completed Date:  2006-11-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806082     Medline TA:  Arch Histol Cytol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  163-71     Citation Subset:  IM    
Department of Oral Anatomy, School of Dentistry, Iwate Medical University, Morioka, Japan.
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MeSH Terms
Adipocytes / cytology*
Cell Differentiation / physiology*
Cell Proliferation
Cells, Cultured
Chick Embryo
Chondrocytes / cytology*
Culture Media / chemistry*
Fatty Acids / analysis,  pharmacology*
Microscopy, Electron, Transmission
Serum / chemistry
Reg. No./Substance:
0/Culture Media; 0/Fatty Acids

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