| In vitro activation of murine peritoneal macrophages by recombinant YopJ: Production of nitric oxide, proinflammatory cytokines and chemokines. | |
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MedLine Citation:
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PMID: 20888665 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Recently it was reported that 3μg/ml of recombinant YopJ induced apoptosis in murine peritoneal macrophages in vitro. However, in this study, we report the activation of murine peritoneal macrophages in vitro on treatment with sub-apoptotic dose of recombinant YopJ protein (1μg/ml). The activation involves enhanced production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), IL-12, and IL-6. Production of NO and IL-6 was found to peak at 24h of rYopJ treatment, whereas IL-12 and IFN-γ production peaked at 18h of rYopJ treatment. Increased mRNAs expression of nitric oxide, IL-12, IL-6 and IFN-γ molecules, was also observed in rYopJ-treated macrophages by RT-PCR. rYopJ induced the enhanced activity of protein tyrosine kinases which was inhibited by pharmacological inhibitor genestein, wortmanin and H-7 suggesting the role of tyrosine kinases, PI3K and PKC in the above process. rYopJ also induced increased enhanced production chemokines MIP-1α, MCP-1, and RANTES in macrophages. Significantly, increased expression of TLR-2, TLR-6, MyD 88 and IRAK-1 was also observed by immunoblotting in rYopJ-treated macrophages. rYopJ induced production of NO, TNF-α and IL-6 was significantly inhibited in macrophages pretreated with pharmacological inhibitor wortmanin, genestein and H-7 demonstrating the probable involvement of protein tyrosine kinases in the above process. |
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Authors:
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Ajit Sodhi; Ashok Kumar Pandey |
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Publication Detail:
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Type: Journal Article Date: 2010-08-19 |
Journal Detail:
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Title: Immunobiology Volume: 216 ISSN: 1878-3279 ISO Abbreviation: Immunobiology Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8002742 Medline TA: Immunobiology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 358-66 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier GmbH. All rights reserved. |
Affiliation:
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School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi 221005, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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