Document Detail

In vitro PGF2alpha production by endometrium and corpus luteum explants from pregnant and nonpregnant diestrus bitches and placental explants from pregnant bitches.
MedLine Citation:
PMID:  16469369     Owner:  NLM     Status:  MEDLINE    
To better understand the process of slow luteal regression of the nonpregnant cycle in dogs and the acute luteolysis that occurs prepartum, the present study investigated in vitro PGF2alpha production by the endometrium, corpus luteum and placental explants obtained at known times of the cycle from pregnant bitches (days 63, 64 and immediately postpartum; day 0 = estimated day of the ovulatory LH surge) and from nonpregnant diestrus bitches (approximately days 65, 75 and 85). Both basal PGF2alpha production and its production in the presence of the protein kinase C (PKC) stimulator 12,13-phorbol dibutyrate (PDBu) were determined. For PDBu-supplemented incubations, mean PGF2alpha production (pg/mL/mg/6 h) by endometrium explants of the nonpregnant bitches in late diestrus was highest on day 65 (205 +/- 87) and reduced to low levels (38 +/- 17 and 11 +/- 11) on days 75 and 85, respectively. The production by corpus luteum explants from these bitches was significantly less on day 65 (46 +/- 14) than that of the day 65 endometrium explants, and was slightly increased on day 85 (103 +/- 52). The corresponding mean PGF2alpha production by the endometrium explants of pregnant bitches was on average much greater (i.e., two to three-fold) compared to nonpregnant bitches (P < 0.01) and involved high concentrations at day 64 (1523 +/- 467) and postpartum, compared to somewhat lower levels on day 63 (830+/-65); luteal PGF production (165 +/- 4) was also higher than in nonpregnant bitches around day 65. For pregnant bitches, PGF production per gram of tissue in the endometrium explants was greater than for the CL or placenta explants (180 +/- 37). Therefore, the endometrium of the pregnant bitch has an increased capability to produce PGF2alpha immediately prepartum, which on a tissue weight basis, exceeds that of either corpora lutea or the placenta. However, assuming a larger mass of placental tissue in vivo, we inferred that the placenta may contribute substantially to peripheral PGF concentrations.
Marcelo Rezende Luz; Cláudia Maria Bertan; Mario Binelli; Maria Denise Lopes
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-15
Journal Detail:
Title:  Theriogenology     Volume:  66     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-18     Completed Date:  2006-11-14     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1442-7     Citation Subset:  IM    
CCA, Department of Animal Science and Rural Economy, Federal University of Espírito Santo-UFES, Alto Universitário, Caixa Postal 16, CEP 29.500-000, Alegre, ES, Brazil.
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MeSH Terms
Corpus Luteum / drug effects,  metabolism*
Diestrus / drug effects,  metabolism
Dinoprost / biosynthesis*
Dogs / metabolism*
Endometrium / drug effects,  metabolism*
Enzyme Activators / pharmacology
Hybridization, Genetic
Phorbol 12,13-Dibutyrate / pharmacology
Placenta / drug effects,  metabolism*
Protein Kinase C / metabolism
Tissue Culture Techniques
Reg. No./Substance:
0/Enzyme Activators; 37558-16-0/Phorbol 12,13-Dibutyrate; 551-11-1/Dinoprost; EC Kinase C

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