Document Detail


In utero lung growth of fetal sheep with diaphragmatic hernia and tracheal stenosis.
MedLine Citation:
PMID:  8632268     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The observation that tracheal ligation produces pulmonary hyperplasia even in animals with surgically induced diaphragmatic hernia (DH) has led to rapid application of the technique to human fetuses with DH. The aim of this study was to determine how rapidly fetal lung volume increases after creation of a high-grade tracheal stenosis in fetal sheep with surgically created DH. Twenty-three fetal sheep were prepared with a left thoracotomy at 90 days' gestational. Six had creation of a DH with tracheal stenosis (DHTS) over an 18-gauge cannula, which was then removed. Ten had DH alone, and seven control animals (CT) had a thoracotomy without DH. Thirty days later, vascular and tracheal loop catheters were inserted in all animals and tunneled out the ewes' flank. Between 125 and 140 days' gestation, lung volumes and lung liquid production were measured in awake, unanesthetized animals using a standard double-marker dilution technique. Average lung volumes (in milliliters) were 150.9 +/- 13.9 for CT, 29.3 +/- 4.4 for DH, and 414.5 +/- 88 for DHTS (p < 0.01). Mean lung liquid production varied from 6.00 +/- 2.23 mL/h in DH animals before 130 days to 16.69 +/- 8.29 mL/h in DHTS animals after 135 days' gestation. DH animals had lower lung liquid production (8.51 +/- 1.4 mL/h) than CT (12.4 +/- 0.8 mL/h) or DHTS animals (12.4 +/- 2.2 mL/h)(P < .01). The rate constant gamma (h-1) for lung liquid production was significantly higher in DH animals than in either CT or DHTS animals (P < .01). Tracheal stenosis in this model causes rapid lung growth before 130 days' gestation. The authors speculate that short periods of incomplete stenosis might reverse the pulmonary hypoplasia associated with DH. To achieve this goal, the timing and duration of treatment and the optimal degree of stenosis must be defined.
Authors:
E A Beierle; M R Langham; S Cassin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  31     ISSN:  0022-3468     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-06-28     Completed Date:  1996-06-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  141-6; discussion 146-7     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Florida College of Medicine, Gainesville, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Embryonic and Fetal Development
Extravascular Lung Water / physiology
Gestational Age
Hernia, Diaphragmatic / congenital,  embryology*
Hyperplasia
Ligation
Lung / embryology*,  pathology
Lung Volume Measurements
Multivariate Analysis
Sheep
Tracheal Stenosis / embryology*
Grant Support
ID/Acronym/Agency:
HL-10834/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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