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In this issue: Biotechnology Journal 2/2010.
MedLine Citation:
PMID:  20151449     Owner:  NLM     Status:  In-Data-Review    
BARLEY, A GREEN LIGAND FACTORY: Erlendsson et al., Biotechnol. J. 2009, 5, 163-171Growth factors are commonly used as cell culture supplements and might gain even more importance in stem cell research, tissue engineering and regenerative medicine or as human therapeutics. Recombinant growth factors are commercially produced in bacteria, yeast, insect and mammalian cells. While these systems are efficient, there is still a need for serum and endotoxin free production systems. Therefore, researchers from Island and co-workers produced biologically active recombinant human Flt3 ligand in transgenic barley seeds. This ligand can then be purified with a tag using immobilized metal ion affinity chromatography (IMAC). The Flt3 is posttranslationally modified and exhibits comparable biological activity to commercial Flt3 ligand. This study demonstrates that plant molecular farming is a viable approach for the bioproduction of human-derived growth factors. TRANSGENIC CROP SAFETY: Herman et al., Biotechnol. J. 2009, 5, 172-182To evalutate the safety of transgenic crops one can analyse the composition of the transgenic crop and compare it to the parent plant or seed. If the composition of nutrients is found to be equivalent to that of non-transgenic variant that is considered safe, then further safety assessment of the transgenic crop can focus solely on the intended modification, like the expression of a transgenic protein. Statistical methods that are used in clinical medicine to compare new generic drugs with brand-name drugs can then be applied to evaluate the equivalence. However, commonly used equivalence limits are shown to be a poor model for comparing transgenic crops with an array of reference crop varieties. Researchers from Dow AgroSciences LLC (USA) suggest an alternate model applied to corn, cotton and soybean seed samples. ADVANCED BIOFUELS FROM MICROBES: Peralta-Yahya and Keasling, Biotechnol. J. 2009, 5, 147-162The climate change and energy security are the major challenges of current times. Biofuels produced from renewable resourses are a cost-effective alternative. Ethanol produced by microorganisms is currently the major biofuel in the transportation sector. However, ethanol's corrosivity and hygroscopicity make it incompatible with existing fuel storage and distribution infrastructure and limits its economic use. Advanced biofuels, such as long chain alcohols and isoprenoid- and fatty acid-based biofuels, have physical properties that more closely resemble petroleum-derived fuels. Therefore, they are attractive candidates for the replacement of petroleum-derived fuels. Authors from the University of California, Berkeley, review recent developments in the engineering of metabolic pathways for the production of advanced biofuels by microorganisms, most importantly Escherichia coli and Saccharomyces cerevisiae.
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology journal     Volume:  5     ISSN:  1860-7314     ISO Abbreviation:  Biotechnol J     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265833     Medline TA:  Biotechnol J     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  136     Citation Subset:  IM    
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