Document Detail


In situ proteo-metabolomics reveals metabolite secretion by the acid mine drainage bio-indicator, Euglena mutabilis.
MedLine Citation:
PMID:  22237547     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Euglena mutabilis is a photosynthetic protist found in acidic aquatic environments such as peat bogs, volcanic lakes and acid mine drainages (AMDs). Through its photosynthetic metabolism, this protist is supposed to have an important role in primary production in such oligotrophic ecosystems. Nevertheless, the exact contribution of E. mutabilis in organic matter synthesis remains unclear and no evidence of metabolite secretion by this protist has been established so far. Here we combined in situ proteo-metabolomic approaches to determine the nature of the metabolites accumulated by this protist or potentially secreted into an AMD. Our results revealed that the secreted metabolites are represented by a large number of amino acids, polyamine compounds, urea and some sugars but no fatty acids, suggesting a selective organic matter contribution in this ecosystem. Such a production may have a crucial impact on the bacterial community present on the study site, as it has been suggested previously that prokaryotes transport and recycle in situ most of the metabolites secreted by E. mutabilis. Consequently, this protist may have an indirect but important role in AMD ecosystems but also in other ecological niches often described as nitrogen-limited.
Authors:
David Halter; Florence Goulhen-Chollet; Sébastien Gallien; Corinne Casiot; Jérôme Hamelin; Françoise Gilard; Dimitri Heintz; Christine Schaeffer; Christine Carapito; Alain Van Dorsselaer; Guillaume Tcherkez; Florence Arsène-Ploetze; Philippe N Bertin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-12
Journal Detail:
Title:  The ISME journal     Volume:  6     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-19     Completed Date:  2013-02-26     Revised Date:  2013-07-02    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  1391-402     Citation Subset:  IM    
Affiliation:
UMR7156 Université de Strasbourg/CNRS, Génétique Moléculaire, Génomique et Microbiologie, Département Micro-organismes, Génomes, Environnement, Strasbourg, France.
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism
Ecosystem*
Euglena / isolation & purification*,  metabolism*
France
Metabolomics
Mining*
Photosynthesis
Chemical
Reg. No./Substance:
0/Acids
Comments/Corrections

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