Document Detail

In situ nick translation of meiotic chromosomes to demonstrate homologous heterochromatin heterogeneity.
MedLine Citation:
PMID:  18469986     Owner:  NLM     Status:  In-Data-Review    
The in situ nick translation procedure performed on fixed meiotic chromosomes partially cleaved with restriction endonucleases shows a different staining of homologous heterochromatic regions, which could be explained through a differential restriction endonuclease cleavage. Mutations occurring before massive tandem duplication and involving those DNA motifs that produce these heterochromatic blocks, together with the absence of DNA recombination that characterizes these particular regions, could explain the observed results. This method for chromosome labelling is most useful to demonstrate a certain level of heterochromatin heterogeneity that is present in the genome of living species but remained cryptic to other techniques that are also able to induce longitudinal differentiation of the chromosomes.
J de L Torre; C López-Fernández; P Herrero; J Gosálvez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Genome / National Research Council Canada = Génome / Conseil national de recherches Canada     Volume:  36     ISSN:  0831-2796     ISO Abbreviation:  Genome     Publication Date:  1993 Apr 
Date Detail:
Created Date:  2008-05-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8704544     Medline TA:  Genome     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  268-70     Citation Subset:  -    
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