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In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states.
MedLine Citation:
PMID:  21766287     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Current state of the art to determine the viability of animal cell suspension cultures is based on sampling and subsequent counting using specific staining assays. We demonstrate for the first time a noninvasive in situ imaging cytometry capable of determining the statistics of a morphologic transition during cell death in suspension cultures. To this end, we measure morphometric inhomogeneity - defined as information entropy - in cell in situ micrographs. We found that the cells are partitioned into two discrete entropy states broadened by phenotypical variability. During the normal course of a culture or by inducing cell death, we observe the transition of cells between these states. As shown by comparison with ex situ diagnostics, the entropy transition happens before or while the cytoplasmatic membrane is loosing its ability to exclude charged dyes. Therefore, measurement of morphometric inhomogeneity constitutes a noninvasive assessment of viability in real time. Biotechnol. Bioeng. © 2011 Wiley Periodicals, Inc.
Authors:
Philipp Wiedemann; Jean S Guez; Hans B Wiegemann; Florian Egner; Juan C Quintana; Diego Asanza-Maldonado; Marcos Filipaki; Jeff Wilkesman; Christian Schwiebert; Jean P Cassar; Pascal Dhulster; Hajo Suhr
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-15
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  -     ISSN:  1097-0290     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Affiliation:
At present: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney 2052, Australia; Mannheim University of Applied Sciences, Paul-Wittsack-Str.10, D-68163 Mannheim, Germany. p.wiedemann@hs-mannheim.de.
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