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In situ measurements of external pH and optical density oscillations in Dictyostelium discoideum aggregates.
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MedLine Citation:
PMID:  3009489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In situ measurements of extracellular pH by means of microelectrodes and in situ measurements of optical density were performed on aggregating cells of Dictyostelium discoideum. Early aggregation stage AX2 cells showed sinusoidal pH oscillations, which could be inhibited by the specific relay inhibitor caffeine, indicating that they were coupled to cAMP oscillations. Sometimes biphasic pH oscillations were found, which can be explained by the superposition of two harmonic pH oscillations. These harmonic oscillations might arise by gating of the cAMP signal; a part of the cells respond to every cAMP signal and another subpopulation to every second cAMP pulse. Late aggregation-stage cells showed complex changes of the extracellular pH, which could be inhibited by caffeine. Optical density measurements of wave propagation in aggregation streams of HG220 also revealed gating behavior. In addition to sinusoidal optical density oscillations, biphasic and still more complex oscillations were observed.
Authors:
K Gottmann; C J Weijer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  102     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-06-12     Completed Date:  1986-06-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1623-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Aggregation
Chemotaxis
Cyclic AMP / physiology
Dictyostelium / cytology*,  physiology
Extracellular Space / physiology
Hydrogen-Ion Concentration*
Periodicity
Spectrophotometry
Chemical
Reg. No./Substance:
60-92-4/Cyclic AMP
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1986
Volume: 102 Issue: 5
First Page: 1623 Last Page: 1629
ID: 2114235
Publisher Id: 86196236
PubMed Id: 3009489

In situ measurements of external pH and optical density oscillations in Dictyostelium discoideum aggregates


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