| In situ measurements of external pH and optical density oscillations in Dictyostelium discoideum aggregates. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 3009489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In situ measurements of extracellular pH by means of microelectrodes and in situ measurements of optical density were performed on aggregating cells of Dictyostelium discoideum. Early aggregation stage AX2 cells showed sinusoidal pH oscillations, which could be inhibited by the specific relay inhibitor caffeine, indicating that they were coupled to cAMP oscillations. Sometimes biphasic pH oscillations were found, which can be explained by the superposition of two harmonic pH oscillations. These harmonic oscillations might arise by gating of the cAMP signal; a part of the cells respond to every cAMP signal and another subpopulation to every second cAMP pulse. Late aggregation-stage cells showed complex changes of the extracellular pH, which could be inhibited by caffeine. Optical density measurements of wave propagation in aggregation streams of HG220 also revealed gating behavior. In addition to sinusoidal optical density oscillations, biphasic and still more complex oscillations were observed. |
| | |
Authors:
|
K Gottmann; C J Weijer |
Related Documents
:
|
17637359 - Bacterial swarming: a re-examination of cell-movement patterns. 7815969 - The malignant stem cell. 915429 - Differences in rotation-mediated aggregation between wild-type and homozygous brachyury... 9398679 - Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesi... 10962479 - Redundancy of lamellipodia in locomoting walker carcinosarcoma cells. 7388899 - Motility, linear arrangement and cell-to-cell contact of myogenic cells prior to fusion. 9721089 - Death receptors: signaling and modulation. 16138329 - Spatially controlled co-culture of neurons and glial cells. 10839359 - A developmentally regulated switch directs regenerative growth of schwann cells through... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of cell biology Volume: 102 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1986 May |
Date Detail:
|
Created Date: 1986-06-12 Completed Date: 1986-06-12 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1623-9 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Aggregation Chemotaxis Cyclic AMP / physiology Dictyostelium / cytology*, physiology Extracellular Space / physiology Hydrogen-Ion Concentration* Periodicity Spectrophotometry |
| Chemical | |
Reg. No./Substance:
|
60-92-4/Cyclic AMP |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 5 Year: 1986 Volume: 102 Issue: 5 First Page: 1623 Last Page: 1629 ID: 2114235 Publisher Id: 86196236 PubMed Id: 3009489 |
| In situ measurements of external pH and optical density oscillations in Dictyostelium discoideum aggregates | |
Article Categories:
|
|
Previous Document: Biological activity of N-nitrosodiethanolamine and of potential metabolites which may arise after ac...
Next Document: Liposome delivery of cyclic AMP-dependent protein kinase inhibitor into intact cells: specific block...