| In situ mapping of urease-positive areas in porcine gastric mucosa. | |
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MedLine Citation:
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PMID: 7476562 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Urease activity is a feature of gastric helicobacters, and its abundant production provides an indirect means of detecting their colonization. A method for mapping urease-positive areas directly on the gastric mucosa was developed, and 57.8% of pigs had evidence of Helicobacter colonization based on urease assay. Moreover, 89.2% of urease-positive pigs had gastritis, confirming that the known association found in man between Helicobacter and gastritis was found also in pigs. The proposed urease assay allowed detection of all urease-positive areas on the gastric mucosa, thus overcoming the biopsy sampling problems derived from the patchy distribution of helicobacters. In this way, gastric mucosa specimens from urease-positive areas could be usefully utilized for culture attempts and for microscopic examination. |
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Authors:
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G M Grasso; M L Sammarco; G Ripabelli; A Ruberto; G Iannitto |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microbios Volume: 82 ISSN: 0026-2633 ISO Abbreviation: Microbios Publication Date: 1995 |
Date Detail:
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Created Date: 1995-12-21 Completed Date: 1995-12-21 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207257 Medline TA: Microbios Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 245-9 Citation Subset: IM |
Affiliation:
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Department of Animal, Plant and Environmental Sciences, Faculty of Agriculture, University of Molise, Campobasso, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Gastric Mucosa / microbiology* Gastritis / microbiology, veterinary Helicobacter / enzymology* Swine Urease / metabolism* |
| Chemical | |
Reg. No./Substance:
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EC 3.5.1.5/Urease |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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