Document Detail

In situ magnetic separation and immobilization of dibenzothiophene-desulfurizing bacteria.
MedLine Citation:
PMID:  19541480     Owner:  NLM     Status:  MEDLINE    
In situ cell separation and immobilization of bacterial cells for biodesulfurization were developed by using superparamagnetic Fe(3)O(4) nanoparticles (NPs). The Fe(3)O(4) NPs were synthesized by coprecipitation followed by modification with ammonium oleate. The surface-modified NPs were monodispersed and the particle size was about 13 nm with 50.8 emu/g saturation magnetization. After adding the magnetic fluids to the culture broth, Rhodococcus erythropolis LSSE8-1 cells were immobilized by adsorption and then separated with an externally magnetic field. The maximum amount of cell mass adsorbed was about 530 g dry cell weight/g particles to LSSE8-1 cells. Analysis showed that the nanoparticles were strongly absorbed to the surface and coated the cells. Compared to free cells, the coated cells not only had the same desulfurizing activity but could also be easily separated from fermentation broth by magnetic force. Based on the adsorption isotherms and Zeta potential analysis, it was believed that oleate-modified Fe(3)O(4) NPs adsorbed bacterial cells mainly because of the nano-size effect and hydrophobic interaction.
Yu-Guang Li; Hong-Shuai Gao; Wang-Liang Li; Jian-Min Xing; Hui-Zhou Liu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-21
Journal Detail:
Title:  Bioresource technology     Volume:  100     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-07-20     Completed Date:  2009-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  5092-6     Citation Subset:  IM    
Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100190, PR China.
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MeSH Terms
Bacteria / cytology*,  isolation & purification*
Biodegradation, Environmental
Cells, Immobilized
Ferrosoferric Oxide / metabolism
Magnetics / methods*
Metal Nanoparticles
Sulfur / isolation & purification*
Thiophenes / isolation & purification*
Reg. No./Substance:
0/Thiophenes; 1317-61-9/Ferrosoferric Oxide; 132-65-0/dibenzothiophene; 7704-34-9/Sulfur

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