Document Detail

In situ labeling and imaging of cellular protein via a bi-functional anticancer aptamer and its fluorescent ligand.
MedLine Citation:
PMID:  22840709     Owner:  NLM     Status:  In-Data-Review    
In this article, we reported a novel approach for in situ labeling and imaging HeLa cancer cells utilizing a bifunctional aptamer (AS1411) and its fluorescent ligand, protoporphyrin IX (PPIX). In the presence of potassium ion, AS1411 folded to G-quadruplex structure, binded fluorescent ligand (PPIX) with fluorescent enhancement, and targeted the nucleolin overexpressed by cancer cells. Consequently, bioimaging of cancer cells specifically were realized by laser scanning confocal microscope. The bioimaging strategy with AS1411-PPIX complex was capable to distinguish HeLa cancer cells from normal cells unambiguously, and fluorescence imaging of cancer cells was also realized in human serum. Moreover, the bioimaging method was very facile, effective and need not any covalent modification. These results illustrated that the useful approach can provide a novel clue for bioimaging based on non-covalent bifunctional aptamer in clinic diagnosis.
Jun Ai; Tao Li; Bingling Li; Yuanhong Xu; Dan Li; Zuojia Liu; Erkang Wang
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Publication Detail:
Type:  Journal Article     Date:  2012-07-05
Journal Detail:
Title:  Analytica chimica acta     Volume:  741     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-07-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  93-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China; Graduate School of the Chinese Academy of Sciences, Beijing 100039, China.
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