| In situ hybridization using biotinylated probes. An evaluation of different detection systems. | |
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MedLine Citation:
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PMID: 2473454 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In situ hybridization with biotin-labelled DNA probes is a powerful tool for the detection of viral sequences in infected tissues. However, sensitivity is low when compared to radiolabelled probes. In order to evaluate the impact on staining results of the detection system applied, we have tested various reagents. In our hands the sequential application of streptavidin and biotinylated alkaline phosphatase or development with a monoclonal anti-biotin antibody and the APAAP method gave consistently the best results. Furthermore, nitro blue tetrazolium/bromochloroindolyphosphate seems to be most suitable as a substrate for the alkaline phosphatase in this case. Use of other reagents, especially of a streptavidin-biotinylated alkaline phosphatase complex, resulted in a significantly lower staining intensity. |
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Authors:
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G Niedobitek; T Finn; H Herbst; H Stein |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pathology, research and practice Volume: 184 ISSN: 0344-0338 ISO Abbreviation: Pathol. Res. Pract. Publication Date: 1989 Mar |
Date Detail:
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Created Date: 1989-08-22 Completed Date: 1989-08-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7806109 Medline TA: Pathol Res Pract Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 343-8 Citation Subset: IM |
Affiliation:
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Department of Pathology, Klinikum Steglitz, Freie Universität Berlin, FRG. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism Antibodies, Monoclonal / diagnostic use, immunology Avidin / diagnostic use Bacterial Proteins / diagnostic use, metabolism Biotin / diagnostic use*, immunology DNA Probes* Gold / diagnostic use Humans Immunoenzyme Techniques Nucleic Acid Hybridization* Staining and Labeling / standards Streptavidin |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Bacterial Proteins; 0/DNA Probes; 1405-69-2/Avidin; 58-85-5/Biotin; 7440-57-5/Gold; 9013-20-1/Streptavidin; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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