| In situ hybridization mapping and restriction fragment length polymorphism analysis of the porcine albumin (ALB) and transferrin (TF) genes. | |
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MedLine Citation:
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PMID: 8101068 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In situ hybridization analyses were conducted on porcine metaphase chromosomes using porcine liver albumin (ALB) and transferrin (TF) cDNA probes. The ALB gene was assigned to the q12 band of chromosome 8 and the TF gene to the q31 band of chromosome 13. For the latter, a statistically significant secondary peak was observed on the 6p15 band. However, the TF probe predominantly hybridized to the 13q31 band, indicating that this band is the most likely site of the TF gene. Since the TF gene belongs to linkage group V, this linkage group can now be assigned to chromosome 13. The TF and ALB probes were also used for restriction fragment length polymorphism (RFLP) analysis. A screening of 10 unrelated animals revealed TaqI RFLPs for both ALB and TF. Family studies indicated that the ALB and TF polymorphisms were controlled by three and two alleles, respectively. |
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Authors:
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B P Chowdhary; M Johansson; R Chaudhary; H Ellegren; F Gu; L Andersson; I Gustavsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Animal genetics Volume: 24 ISSN: 0268-9146 ISO Abbreviation: Anim. Genet. Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-08-09 Completed Date: 1993-08-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8605704 Medline TA: Anim Genet Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 85-90 Citation Subset: IM |
Affiliation:
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Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alleles Animals Autoradiography / veterinary Chromosome Banding / veterinary Chromosome Mapping / veterinary DNA Probes Genes, Dominant In Situ Hybridization / veterinary Linkage (Genetics) Metaphase Polymorphism, Restriction Fragment Length* Serum Albumin / genetics* Swine / genetics* Transferrin / genetics* |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Serum Albumin; 11096-37-0/Transferrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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