| In situ hybridization of human kidney tissue reveals cell-type-specific expression of the O6-methylguanine-DNA methyltransferase gene. | |
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MedLine Citation:
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PMID: 1547538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cell-type-specific expression of human O6-methylguanine-DNA methyltransferase (O6-MT) was determined in paraffin-embedded sections of human kidney. A 39 base oligomer complementary to O6-MT cDNA was labeled with digoxigenin and visually detected in situ using an alkaline phosphatase-conjugated anti-digoxigenin antibody. This allowed direct determination of O6-MT-specific mRNA levels, while simultaneously identifying the structures and cell types in the kidney section. Expression of O6-MT was high in distal tubular and glomerular epithelial cells and low in the cells of the Bowman's capsule, collecting and proximal tubular cells. Hybridization of the oligomer was specific to RNA, since RNase and not DNase eliminated the signal. Expression was uniform in all the cell types, except the glomerular cells exhibited varying levels of high intensity. Cell-specific expression was constant between tissue sections from the same and different kidney tissues. These data may help explain the differential response of various cell types to alkylating agents. |
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Authors:
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G Wani; A A Wani; S M D'Ambrosio |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carcinogenesis Volume: 13 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-04-17 Completed Date: 1992-04-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 463-8 Citation Subset: IM |
Affiliation:
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Department of Radiology, College of Medicine, Ohio State University, Columbus 43210. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA Repair* Deoxyribonucleases / diagnostic use Fetus Guanosine / analogs & derivatives*, analysis Humans Kidney / chemistry*, cytology Methyltransferases / genetics* Nucleic Acid Hybridization O(6)-Methylguanine-DNA Methyltransferase RNA, Messenger / analysis* Ribonucleases / diagnostic use |
| Grant Support | |
ID/Acronym/Agency:
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ES2388/ES/NIEHS NIH HHS; R01-ES3101/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 118-00-3/Guanosine; 7803-88-5/6'-O-methylguanosine; EC 2.1.1.-/Methyltransferases; EC 2.1.1.63/O(6)-Methylguanine-DNA Methyltransferase; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Ribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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