Document Detail


In situ hybridization detection of light chain mRNA in routine bone marrow trephines from patients with suspected myeloma.
MedLine Citation:
PMID:  2513865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the detection of immunoglobulin light chain mRNA by situ hybridization has been applied to routine bone marrow trephines, from patients suspected of having multiple myeloma. The principle aim was to define monoclonal populations even in cases lacking a paraprotein or an obvious atypical plasma cell proliferation. The expression of light chain mRNA in plasma cells in routine bone marrow trephines from 14 patients with suspected myeloma was studied. Plasma cells in the marrow ranged from 4% to 99%. Clonal populations based on light chain restriction were delineated in 12 patients, using biotinylated probes to kappa and lambda mRNA and visualized using an alkaline phosphatase/fast red naphthol capture method. Normal tonsil and bone marrow trephines were used as controls. In situ hybridization was found to be a specific, safe and rapid technique. It could be applied to both fixed and fresh tissue. It was found to lack the background staining of interstitial immunoglobulin, particularly in sclerotic cases, which is so often encountered with immunocytochemistry. Additionally, this technique will type plasma cell neoplasms (undetected by routine immunocytochemistry) in post-transcriptional block or non-secretory myeloma.
Authors:
N Akhtar; A Ruprai; J H Pringle; I Lauder; S T Durrant
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of haematology     Volume:  73     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-02-16     Completed Date:  1990-02-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  296-301     Citation Subset:  IM    
Affiliation:
Department of Haematology, Leicester Royal Infirmary.
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow / immunology*,  surgery
Humans
Immunoglobulin Light Chains / analysis*
Immunoglobulin kappa-Chains / analysis
Immunoglobulin lambda-Chains / analysis
Multiple Myeloma / immunology*
Nucleic Acid Hybridization
Oligonucleotide Probes
RNA, Messenger / analysis*
Chemical
Reg. No./Substance:
0/Immunoglobulin Light Chains; 0/Immunoglobulin kappa-Chains; 0/Immunoglobulin lambda-Chains; 0/Oligonucleotide Probes; 0/RNA, Messenger

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