Document Detail


In situ hybridization for the detection of cytomegalovirus (CMV) infection. Application of biotinylated CMV-DNA probes on paraffin-embedded specimens.
MedLine Citation:
PMID:  3020777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Five autopsy cases of cytomegalovirus (CMV) infections were studied. Conventional light microscopy disclosed characteristic cytopathic effects in lungs, kidneys, and brain. In one case, electron microscopy was carried out and revealed typical herpesvirus particles. In situ hybridization was done with biotin-labeled CMV-DNA probes and an avidin-alkaline phosphatase detection system. 4/5 cases were observed to contain hybridizing cells in different organs. Intensity of hybridization was related to the severity of CMV infection, roughly estimated by counting cytomegalic cells. In addition to cytomegalic cells, a high number of normal-looking epithelial and mesenchymal cell types were positive. These latter cells showed nuclear hybridizations in contrast to cytomegalic cells which hybridized both within the nuclei and the cell bodies. This modified in situ hybridization procedure is a rapid and valuable tool for the detection and final demonstration of virus infection, and will be of particular help for the examination of paraffin-embedded specimens.
Authors:
T Löning; K Milde; H D Foss
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virchows Archiv. A, Pathological anatomy and histopathology     Volume:  409     ISSN:  0174-7398     ISO Abbreviation:  Virchows Arch A Pathol Anat Histopathol     Publication Date:  1986  
Date Detail:
Created Date:  1986-10-30     Completed Date:  1986-10-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8302198     Medline TA:  Virchows Arch A Pathol Anat Histopathol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  777-90     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Biotin / diagnostic use
Cytomegalovirus / genetics*
Cytomegalovirus Infections / diagnosis*,  pathology
DNA, Viral / genetics*
Histological Techniques
Humans
Infant, Newborn
Male
Middle Aged
Nucleic Acid Hybridization*
Chemical
Reg. No./Substance:
0/DNA, Viral; 58-85-5/Biotin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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