Document Detail

In situ expression of a B7-like adhesion molecule on keratinocytes from human epidermis.
MedLine Citation:
PMID:  7693826     Owner:  NLM     Status:  MEDLINE    
The B7 adhesion molecule, a member of the immunoglobulin superfamily, has previously been identified primarily on cells of hematopoietic origin. Because B7 has been shown to facilitate interactions with T cells and because cells of the epidermis are proficient at binding and activating T lymphocytes, studies were performed to determine whether B7 was expressed in human epidermis. A subpopulation of brightly staining B7-positive cells was observed in situ in normal human epidermis. Flow-cytometric examination of epidermal cell suspensions that had been cultured for 24 h or longer demonstrated that between 10 and 40% of cells expressed B7 or a closely related antigen. Immunoelectron microscopy, double-staining procedures, and examination of epidermal suspensions depleted of Langerhans cells all confirmed that the B7-positive cells were keratinocytes. These studies identify human epidermal keratinocytes, a non-hematopoietic cell population, as a cell type capable of expressing a B7-like adhesion molecule.
T E Fleming; W S Mirando; U Trefzer; K A Tubesing; C A Elmets
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  101     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1993 Nov 
Date Detail:
Created Date:  1993-11-26     Completed Date:  1993-11-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  754-8     Citation Subset:  IM    
Department of Dermatology, Case Western Reserve University, Cleveland, Ohio.
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MeSH Terms
Antigens, CD80 / analysis*,  physiology
Cell Adhesion Molecules / analysis*
Epidermis / cytology*
HLA-DR Antigens / analysis
Keratinocytes / chemistry*
Microscopy, Immunoelectron
Grant Support
Reg. No./Substance:
0/Antigens, CD80; 0/Cell Adhesion Molecules; 0/HLA-DR Antigens; 0/Suspensions

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