Document Detail

In situ electrochemical monitoring of reactive oxygen and nitrogen species released by single MG63 osteosarcoma cell submitted to a mechanical stress.
MedLine Citation:
PMID:  20577682     Owner:  NLM     Status:  MEDLINE    
The oxidative stress responses of single MG63 osteosarcoma cells submitted to a brief mechanical stress have been investigated by amperometry at platinized carbon fiber electrodes for monitoring and characterizing the nature and the amounts of the various reactive oxygen (ROS) and reactive nitrogen species (RNS) released. It was thus shown that, on average, a single MG63 cell released prominent amounts of reactive nitrogen species (17 fmol NO(*), 6 fmol ONOO(-), and 5 fmol NO(2)(-)) together with a comparatively small quantity of H(2)O(2) (2 fmol). These species resulted from the primary production of 13 fmol for O(2)(*-) and 28 fmol for NO(*) per single cell as reconstructed from the stoichiometries of the ROS and RNS releases. The high NO(*)/H(2)O(2) and NO(*)/O(2)(*-) ratios thus found are perfectly consistent with previous claims that the malignant bone formation ability of the osteosarcoma cells is related to a specific high production of NO* associated to a small one of O(2)(*-).
Ren Hu; Manon Guille; Stéphane Arbault; Chang Jian Lin; Christian Amatore
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-24
Journal Detail:
Title:  Physical chemistry chemical physics : PCCP     Volume:  12     ISSN:  1463-9084     ISO Abbreviation:  Phys Chem Chem Phys     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-20     Completed Date:  2010-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888160     Medline TA:  Phys Chem Chem Phys     Country:  England    
Other Details:
Languages:  eng     Pagination:  10048-54     Citation Subset:  IM    
UMR CNRS-ENS-UPMC 8640 Pasteur and LIA CNRS XiamENS, Département de Chimie, Ecole Normale Supérieure, 75231 Paris, France.
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MeSH Terms
Cell Line, Tumor
Electrochemistry / methods*
Osteosarcoma / metabolism,  pathology*
Oxidative Stress
Reactive Nitrogen Species / metabolism*
Reactive Oxygen Species / metabolism*
Stress, Mechanical*
Reg. No./Substance:
0/Reactive Nitrogen Species; 0/Reactive Oxygen Species

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