Document Detail

In situ distribution of EcoRI methylase and restriction endonuclease in cells of Escherichia coli Bs 5.
MedLine Citation:
PMID:  3034675     Owner:  NLM     Status:  MEDLINE    
Specific IgG antibodies were raised in rabbits against purified EcoRI methylase and restriction endonuclease. Post embedding labeling experiments, using the protein A-gold technique, were made with paraformaldehyde-glutaraldehyde fixed cells, embedded in Lowicryl K4M resin at low temperatures. Labeling with methylase-specific antibodies showed 60-70% of gold particles in the cytoplasm and 30-40% at the cell envelope, whereas the use of restriction enzyme-specific antibodies led to a distribution of 10-30% in the cytoplasm and 70-90% in the cell envelope. The results coincide with the proposed function of the enzymes: in the cytoplasm methylase protects the cells' own DNA from self-destruction, and the restriction endonuclease cuts foreign DNA when entering the cell.
G W Kohring; F Mayer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  216     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-07-16     Completed Date:  1987-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-10     Citation Subset:  IM    
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MeSH Terms
Cell Compartmentation
DNA Restriction Enzymes / metabolism*
Deoxyribonuclease EcoRI
Escherichia coli / enzymology*,  ultrastructure
Gold / diagnostic use
Immunologic Techniques
Methyltransferases / metabolism*
Microscopy, Electron
Site-Specific DNA-Methyltransferase (Adenine-Specific)*
Reg. No./Substance:
7440-57-5/Gold; EC 2.1.1.-/DNA modification methylase EcoRI; EC 2.1.1.-/Methyltransferases; EC DNA-Methyltransferase (Adenine-Specific); EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease EcoRI

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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