Document Detail

In situ distribution of EcoRI methylase and restriction endonuclease in cells of Escherichia coli Bs 5.
MedLine Citation:
PMID:  3034675     Owner:  NLM     Status:  MEDLINE    
Specific IgG antibodies were raised in rabbits against purified EcoRI methylase and restriction endonuclease. Post embedding labeling experiments, using the protein A-gold technique, were made with paraformaldehyde-glutaraldehyde fixed cells, embedded in Lowicryl K4M resin at low temperatures. Labeling with methylase-specific antibodies showed 60-70% of gold particles in the cytoplasm and 30-40% at the cell envelope, whereas the use of restriction enzyme-specific antibodies led to a distribution of 10-30% in the cytoplasm and 70-90% in the cell envelope. The results coincide with the proposed function of the enzymes: in the cytoplasm methylase protects the cells' own DNA from self-destruction, and the restriction endonuclease cuts foreign DNA when entering the cell.
G W Kohring; F Mayer
Related Documents :
19877125 - Cellular and transcriptional responses of yeast to the cleavage of cytosolic trnas indu...
3897845 - On mutation fixation resulting from nitrosoguanidine-induced dna damage in escherichia ...
374385 - Individual proteins are synthesized continuously throughout the escherichia coli cell c...
24152585 - Inhibition of cancer cell proliferation and apoptosis-inducing activity of fungal taxol...
10444575 - Pretreatment with inducers of er molecular chaperones protects epithelial cells subject...
7473815 - Growth inhibition and induction of apoptosis by fenretinide in small-cell lung cancer c...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  216     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-07-16     Completed Date:  1987-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-10     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Compartmentation
DNA Restriction Enzymes / metabolism*
Deoxyribonuclease EcoRI
Escherichia coli / enzymology*,  ultrastructure
Gold / diagnostic use
Immunologic Techniques
Methyltransferases / metabolism*
Microscopy, Electron
Site-Specific DNA-Methyltransferase (Adenine-Specific)*
Reg. No./Substance:
7440-57-5/Gold; EC 2.1.1.-/DNA modification methylase EcoRI; EC 2.1.1.-/Methyltransferases; EC DNA-Methyltransferase (Adenine-Specific); EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease EcoRI

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Insulin stimulates a novel GTPase activity in human platelets.
Next Document:  Absorption mode two-dimensional NOE spectroscopy of exchangeable protons in oligonucleotides.