| In situ detection of superoxide anions within porcine articular cartilage. | |
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MedLine Citation:
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PMID: 10367405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cartilage was isolated from pig articular joints, and the production of reactive oxygen species (ROS) by chondrocytes embedded within the cartilage was assessed by two methods: the reduction of nitro blue tetrazolium and by the use of diaminobenzidine in the presence of manganese ions. Little constitutive generation of ROS was seen, but it could be detected after the addition of the calcium ionophore ionomycin. Further, the response seen was extremely heterogeneous; some cells showed a far greater release of ROS than others. Cells arranged in the columnar arrays of the deep zone were the most active, while those furthest from the cartilage/bone interface (i.e. nearer to the outer face of the cartilage) were unresponsive. Chondrocytes cultured in alginate beads also showed a similar heterogeneity in their response, suggesting that the isolation of these cells and the measurement of ROS production in a population is not representative of the true situation. |
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Authors:
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T S Hiran; P J Moulton; J T Hancock |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: British journal of biomedical science Volume: 55 ISSN: 0967-4845 ISO Abbreviation: Br. J. Biomed. Sci. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1999-06-28 Completed Date: 1999-06-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9309208 Medline TA: Br J Biomed Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 199-203 Citation Subset: IM |
Affiliation:
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Herman B. Wells Center for Pediatric Research, Department of Pediatrics (Hematology/Oncology), James Whitcomb Riley Hospital for Children, Indiana University Medical Center, Indianapolis 46202, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage, Articular / metabolism* Cell Culture Techniques Chondrocytes / metabolism Reactive Oxygen Species / metabolism* Swine |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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