Document Detail


In situ detection of apoptosis in regressing corpus luteum of pregnant sow: evidence of an early presence of DNA fragmentation.
MedLine Citation:
PMID:  8839629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Luteolysis has been shown to be correlated with apoptosis in rats, sheep, and cows. In pigs, apoptosis has already been demonstrated as regards atretic follicles. The present study has been conducted to evaluate whether apoptosis occurs during corpora lutea regression in the pregnant pig and to investigate the temporal relationship between apoptosis and functional luteolysis. The apoptotic process has been studied through the research of oligonucleosome fragmentation by means of classical electrophoresis methods and by in situ detection on histological luteal sections. The latter method allows the identification of apoptosis and the localization of apoptotic cells. Pregnant sows were cloprostenol (PGF2 alpha analog) treated and ovariectomized 0, 6, 12, 24, 48, and 72 hr after treatment. Corpora lutea were utilized for progesterone and DNA extraction and in situ evaluation of apoptosis. Clear evidence of apoptosis was seen earlier with the in situ technique (6 hr for stromal tissue, 12 hr for luteal cells) than with the classical method (24 hr). Apoptosis was, however, apparent after plasma and tissue progesterone had reached basal levels. In conclusion, these results are consistent with the hypothesis that apoptosis occurs during luteolysis in pigs. Moreover, the data obtained with the in situ technique made it possible to identify signs of structural regression in stromal tissue first than in parenchymal cells. A two-stage activation of apoptosis has been discussed to explain structural changes that occur during luteolysis after cloprostenol treatment in swine corpora lutea.
Authors:
M L Bacci; A M Barazzoni; M Forni; G L Costerbosa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  13     ISSN:  0739-7240     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  1996 Jul 
Date Detail:
Created Date:  1996-12-12     Completed Date:  1996-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  361-72     Citation Subset:  IM    
Affiliation:
Department of Veterinary Morphology and Physiology, University of Bologna, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Capillaries / anatomy & histology
Cloprostenol / pharmacology
Corpus Luteum / blood supply,  chemistry*
DNA / analysis
DNA Fragmentation*
Female
Luteolysis*
Ovariectomy
Pregnancy
Progesterone / blood
Swine*
Chemical
Reg. No./Substance:
40665-92-7/Cloprostenol; 57-83-0/Progesterone; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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