Document Detail

In situ detection of HY-specific T cells in acute graft-versus-host disease-affected male skin after gender mismatched stem-cell transplantation.
MedLine Citation:
PMID:  22062805     Owner:  NLM     Status:  Publisher    
HY-specific T cells are presumed to play a role in acute Graft-versus-Host Disease (aGVHD) after female-to-male stem-cell transplantation (SCT). Yet, infiltrates of these T cells in aGVHD-affected tissues have thus far not been shown. Here we report on the validation and application of HLA-A2/HY dextramers for the in situ detection of minor HY-specific T cells in cryopreserved biopsies. We applied the HLA-A2/HY dextramers on cryopreserved skin biopsies from 7 male HLA-A2(pos) pediatric SCT patients with confirmed aGVHD involving the skin. Indeed, the dextramers successfully demonstrated the presence of HY-specific T cells; in skin biopsies of 3 male recipients with female grafts, 68 to 78% of all skin infiltrating CD8(pos) T cells were HY specific. These cells were absent in biopsies collected from gender-matched patient-donor pairs. Although analyzed in a small and heterogeneous patient group, our results strongly support the hypothesis that HY-specific T cells are actively involved in the pathophysiology of aGvHD after gender mismatched SCT.
Yeung-Hyen Kim; Claudia M J M Faaij; Astrid G S van Halteren; Ellen Schrama; Trees A M Dellemijn; Jørgen Schøller; R Maarten Egeler; Stan Pavel; Florry A Vyth-Dreese; Maarten J D van Tol; Els Goulmy; Eric Spierings
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-4
Journal Detail:
Title:  Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation     Volume:  -     ISSN:  1523-6536     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9600628     Medline TA:  Biol Blood Marrow Transplant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, The Netherlands.
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