| In situ dark field microscopy for on-line monitoring of yeast cultures. | |
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MedLine Citation:
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PMID: 17186133 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new-type in situ probe has been developed to acquire dark field images of yeast in bioreactors. It has been derived from an in situ bright field microscope that is able to measure cell density in bioreactors during fermentation processes. The illumination part of the probe has been replaced with a dark field device, in which an aspheric condenser is used, so that high contrast dark field images can be obtained. The technique of second imaging is implemented to improve the sharpness of the images by means of a relay lens. This new in situ probe is expected to enable the evaluation of the cell viability without staining owing to modern image processing. |
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Authors:
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Ning Wei; Jia You; Karl Friehs; Erwin Flaschel; Tim Wilhelm Nattkemper |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-12-21 |
Journal Detail:
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Title: Biotechnology letters Volume: 29 ISSN: 1573-6776 ISO Abbreviation: Biotechnol. Lett. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-19 Completed Date: 2007-09-13 Revised Date: 2008-10-07 |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 373-8 Citation Subset: IM |
Affiliation:
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Bielefeld University, Faculty of Technology, Fermentation Engineering, Bielefeld, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors
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microbiology* Cell Count / instrumentation*, methods Equipment Design Equipment Failure Analysis Image Enhancement / instrumentation, methods* Image Interpretation, Computer-Assisted / methods* Microscopy / instrumentation*, methods Online Systems Yeasts / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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