Document Detail

In situ crosslinkable hyaluronan hydrogels for tissue engineering.
MedLine Citation:
PMID:  14643608     Owner:  NLM     Status:  MEDLINE    
We describe the development of an injectable, cell-containing hydrogel that supports cell proliferation and growth to permit in vivo engineering of new tissues. Two thiolated hyaluronan (HA) derivatives were coupled to four alpha,beta-unsaturated ester and amide derivatives of poly(ethylene glycol) (PEG) 3400. The relative chemical reactivity with cysteine decreased in the order PEG-diacrylate (PEGDA)>>PEG-dimethacrylate>PEG-diacrylamide>PEG-dimethacrylamide. The 3-thiopropanoyl hydrazide derivative (HA-DTPH) was more reactive than the 4-thiobutanoyl hydrazide, HA-DTBH. The crosslinking of HA-DTPH with PEGDA in a molar ratio of 2:1 occurred in approximately 9 min, suitable for an in situ crosslinking applications. The in vitro cytocompatibility and in vivo biocompatibility were evaluated using T31 human tracheal scar fibroblasts, which were suspended in medium in HA-DTPH prior to addition of the PEGDA solution. The majority of cells survived crosslinking and the cell density increased tenfold during the 4-week culture period in vitro. Cell-loaded hydrogels were also implanted subcutaneously in the flanks of nude mice, and after immunohistochemistry showed that the encapsulated cells retained the fibroblast phenotype and secreted extracellular matrix in vivo. These results confirm the potential utility of the HA-DTPH-PEGDA hydrogel as an in situ crosslinkable, injectable material for tissue engineering.
Xiao Zheng Shu; Yanchun Liu; Fabio S Palumbo; Yi Luo; Glenn D Prestwich
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biomaterials     Volume:  25     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:    2004 Mar-Apr
Date Detail:
Created Date:  2003-12-03     Completed Date:  2004-08-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  1339-48     Citation Subset:  IM    
Department of Medicinal Chemistry, The University of Utah, 419 Wakara Way, Suite 205, Salt Lake City, UT 84108-1257, USA.
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MeSH Terms
Biocompatible Materials / chemical synthesis*,  chemistry
Cell Division
Cell Survival
Cells, Cultured
Cross-Linking Reagents / chemistry
Fibroblasts / pathology*
Foreign-Body Reaction / pathology*
Hyaluronic Acid / administration & dosage,  chemistry*
Hydrogels / administration & dosage,  chemistry*
Materials Testing
Mice, Nude
Tissue Engineering / methods*
Grant Support
Reg. No./Substance:
0/Biocompatible Materials; 0/Cross-Linking Reagents; 0/Hydrogels; 9004-61-9/Hyaluronic Acid

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