| In situ chemical aminoacylation with amino acid thioesters linked to a peptide nucleic acid. | |
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MedLine Citation:
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PMID: 15584731 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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tRNA-specific chemical aminoacylation was achieved with nonnatural amino acids. A nonnatural amino acid was activated as a thioester derivative, and the latter was linked through a spacer to the N-terminal of a 9-mer peptide nucleic acid that is complementary to the 3'-terminal region of yeast phenylalanine tRNA. Efficient aminoacylation was observed when the amino acid thioester-spacer-PNA conjugate was mixed with the tRNA. The PNA-assisted aminoacylation was also successful in an Escherichia coli in vitro protein synthesizing system that contained an orthogonalized tRNA. The in situ aminoacylation/in vitro translation gave a mutant protein in which the nonnatural amino acid was incorporated into the position directed by a CGGG 4-base codon/anticodon pair. |
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Authors:
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Keiko Ninomiya; Toshikazu Minohata; Masaki Nishimura; Masahiko Sisido |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 126 ISSN: 0002-7863 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-08 Completed Date: 2005-02-15 Revised Date: 2012-08-01 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 15984-9 Citation Subset: IM |
Affiliation:
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Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acylation Amino Acids / chemistry*, metabolism Computer-Aided Design Escherichia coli / genetics, metabolism Esters / chemistry, metabolism Models, Molecular Nucleic Acid Hybridization Peptide Nucleic Acids / biosynthesis, chemistry* Protein Biosynthesis* RNA, Transfer, Amino Acyl / chemistry*, metabolism Sulfhydryl Compounds / chemistry, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Esters; 0/Peptide Nucleic Acids; 0/RNA, Transfer, Amino Acyl; 0/Sulfhydryl Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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