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In-silico and In-vitro based studies of Streptomyces peucetius CYP107N3 for oleic acid epoxidation.
MedLine Citation:
PMID:  23261061     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Certain members of the cytochromes P450 superfamily metabolize polyunsaturated long-chain fatty acids to several classes of oxygenated metabolites. An approach based on in silico analysis predicted that Streptomyces peucetius CYP107N3 might be a fatty acid-metabolizing enzyme, showing high homology with epoxidase enzymes. Homology modeling and docking studies of CYP107N3 showed that oleic acid can fit directly into the active site pocket of the double bond of oleic acid within optimum distance of 4.6 Å from the Fe. In order to confirm the epoxidation activity proposed by in silico analysis, a gene coding CYP107N3 was expressed in Escherichia coli. The purified CYP107N3 was shown to catalyze C(9)-C(10) epoxidation of oleic acid in vitro to 9,10-epoxy stearic acid confirmed by ESI-MS, HPLC-MS and GC-MS spectral analysis. [BMB Reports 2012; 45(12): 736-741].
Authors:
Saurabh Bhattarai; Narayan Prasad Niraula; Jae Kyung Sohng; Tae-Jin Oh
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  BMB reports     Volume:  45     ISSN:  1976-670X     ISO Abbreviation:  BMB Rep     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101465334     Medline TA:  BMB Rep     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  736-41     Citation Subset:  IM    
Affiliation:
Institute of Biomolecule Reconstruction (IBR), Department of Pharmaceutical Engineering, SunMoon University, Asan 336-708, Korea tjoh3782@sunmoon.ac.kr.
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