Document Detail


In ovo time-lapse analysis after dorsal neural tube ablation shows rerouting of chick hindbrain neural crest.
MedLine Citation:
PMID:  10851129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous analyses of single neural crest cell trajectories have suggested important roles for interactions between neural crest cells and the environment, and amongst neural crest cells. To test the relative contribution of intrinsic versus extrinsic information in guiding cells to their appropriate sites, we ablated subpopulations of premigratory chick hindbrain neural crest and followed the remaining neural crest cells over time using a new in ovo imaging technique. Neural crest cell migratory behaviors are dramatically different in ablated compared with unoperated embryos. Deviations from normal migration appear either shortly after cells emerge from the neural tube or en route to the branchial arches, areas where cell-cell interactions typically occur between neural crest cells in normal embryos. Unlike the persistent, directed trajectories in normal embryos, neural crest cells frequently change direction and move somewhat chaotically after ablation. In addition, the migration of neural crest cells in collective chains, commonly observed in normal embryos, was severely disrupted. Hindbrain neural crest cells have the capacity to reroute their migratory pathways and thus compensate for missing neural crest cells after ablation of neighboring populations. Because the alterations in neural crest cell migration are most dramatic in regions that would normally foster cell-cell interactions, the trajectories reported here argue that cell-cell interactions have a key role in the shaping of the neural crest migration.
Authors:
P Kulesa; M Bronner-Fraser; S Fraser
Related Documents :
9811589 - A role for rhob in the delamination of neural crest cells from the dorsal neural tube.
10655589 - Plasticity in mouse neural crest cells reveals a new patterning role for cranial mesoderm.
9469579 - Developmental pattern of expression of npdc-1 and its interaction with e2f-1 suggest a ...
19127979 - Collective cell migration drives morphogenesis of the kidney nephron.
7620899 - Müller cell survival and proliferation in response to medium conditioned by the retina...
18790399 - Magnetic resonance imaging detects differences in migration between primary and immorta...
12175289 - Contrast agents for confocal microscopy: how simple chemicals affect confocal images of...
15087469 - Foxo1a can alter cell cycle progression by regulating the nuclear localization of p27ki...
22820149 - Anticancer mechanisms of action of two small amphipathic β(2,2)-amino acid derivatives...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  127     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-08-16     Completed Date:  2000-08-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2843-52     Citation Subset:  IM    
Affiliation:
Division of Biology and Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA. kulesa@gg.caltech.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning*
Brain / embryology*
Cell Communication
Cell Movement
Chick Embryo
Image Processing, Computer-Assisted
Microscopy, Confocal
Microscopy, Video
Neural Crest / embryology*,  physiology*
Ovum / physiology*
Time Factors
Grant Support
ID/Acronym/Agency:
HD15527/HD/NICHD NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteog...
Next Document:  An FGF signal from endoderm and localized factors in the posterior-vegetal egg cytoplasm pattern the...