| In ovo DNA immunisation followed by a recombinant fowlpox boost is fully protective to challenge with virulent IBDV. | |
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MedLine Citation:
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PMID: 16621184 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to investigate the potential use of DNA vaccination delivered in ovo for protecting against challenge with infectious bursal disease virus (IBDV). Using a plasmid expressing the beta-galactosidase gene, DNA was successfully delivered to the embryo after in ovo injection and localises to the proventriculus and thymus. The coding sequence for the immunogenic IBDV protein, VP2, was cloned into pCI-neo, creating pCI-Vp2. Complete protection against IBDV was obtained by priming in ovo with pCI-Vp2, followed by boosting with the fowlpox recombinant, fpIBD1, also expressing the VP2 gene. This complete protection was not evident with either of the experimental vaccines on their own. An antibody response was not detected after the prime-boost vaccination, even after chicks had been challenged with IBDV, implying that the DNA prime delivered in ovo stimulated a protective cellular immune response. |
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Authors:
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E A Haygreen; P Kaiser; S C Burgess; T F Davison |
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Publication Detail:
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Type: Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-04-03 |
Journal Detail:
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Title: Vaccine Volume: 24 ISSN: 0264-410X ISO Abbreviation: Vaccine Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-02 Completed Date: 2006-08-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8406899 Medline TA: Vaccine Country: Netherlands |
Other Details:
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Languages: eng Pagination: 4951-61 Citation Subset: IM |
Affiliation:
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Institute for Animal Health, Compton, Berkshire RG20 7NN, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amniotic Fluid
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metabolism Animals Birnaviridae Infections / immunology, prevention & control, veterinary* Chick Embryo Chickens DNA, Recombinant Deoxyribonucleases / metabolism Fowlpox virus / immunology* Immunization, Secondary* Infectious bursal disease virus / immunology*, pathogenicity Poultry Diseases / immunology, prevention & control* RNA, Viral / isolation & purification Vaccines, DNA / administration & dosage, immunology* Viral Vaccines / administration & dosage, immunology* Virulence |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/RNA, Viral; 0/Vaccines, DNA; 0/Viral Vaccines; EC 3.1.-/Deoxyribonucleases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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