Document Detail


In-house SIRAS phasing of the polyunsaturated fatty-acid isomerase from Propionibacterium acnes.
MedLine Citation:
PMID:  16511289     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polyenoic fatty-acid isomerase from Propionibacterium acnes (PAI) catalyzes the double-bond isomerization of linoleic acid to conjugated linoleic acid, which is a dairy- or meat-derived fatty acid in the human diet. PAI was overproduced in Escherichia coli and purified to homogeneity as a yellow-coloured protein. The nature of the bound cofactor was analyzed by absorption and fluorescence spectroscopy. Single crystals of PAI were obtained in two crystal forms. Cubic shaped crystals belong to space group I2(1)3, with a unit-cell parameter of 160.4 A, and plate-like crystals belong to the monoclinic space group C2, with unit-cell parameters a = 133.7, b = 60.8, c = 72.2 A, beta = 115.8 degrees. Both crystal forms contain one molecule per asymmetric unit and diffract to a resolution of better than 2.0 A. Initial phases were obtained by SIRAS from in-house data from a cubic crystal that was soaked with an unusually low KI concentration of 0.25 M.
Authors:
Alena Liavonchanka; Ellen Hornung; Ivo Feussner; Markus Rudolph
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-27
Journal Detail:
Title:  Acta crystallographica. Section F, Structural biology and crystallization communications     Volume:  62     ISSN:  1744-3091     ISO Abbreviation:  Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-03-02     Completed Date:  2006-05-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  101226117     Medline TA:  Acta Crystallogr Sect F Struct Biol Cryst Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  153-6     Citation Subset:  IM    
Affiliation:
Department of Plant Biochemistry, Georg-August-Universität, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry*,  genetics,  isolation & purification
Carbon-Carbon Double Bond Isomerases / chemistry*,  genetics,  isolation & purification
Cloning, Molecular
Crystallization
Crystallography, X-Ray
Propionibacterium acnes / enzymology*
Scattering, Radiation
Spectrometry, Fluorescence
Chemical
Reg. No./Substance:
0/Bacterial Proteins; EC 5.3.3.-/Carbon-Carbon Double Bond Isomerases; EC 5.3.3.-/polyenoic fatty acid isomerase
Comments/Corrections

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